Yugenraj Navaneethan

5 Questions 5 Answers 0 Followers

Questions related from Yugenraj Navaneethan

Statistical analyses for biofilm assays?

Hi I performed 24 hr biofilm formation assay (a total of nine replicates for each bacterial strain). And from the OD570nm, I classify them according to their biofilm forming capacity by using the...

23 August 2019 586 2 View

Any threshold value (for enzyme activity) that is required/ sufficient for microbial spoilage of food?

Hello Spoilage causing microorganisms (yeast, mold, bacteria) cause undesirable changes in food through their enzymatic actions. Is there a threshold value for enzyme activity (can be for any...

14 May 2019 7,697 1 View

What is the right azocasein assay method?

Hi I would like to know how to construct standard curve for azocasein assay? I know that tyrosine is usually used for the standard curve of protease assay. The tyrosine standard measured...

15 April 2019 9,734 3 View

Question regarding PCR & bands on agarose gel?

I'm trying to detect hblA gene by using the PCR method as described by the journal"Enterotoxigenic Profiles of Food-Poisoning and Food-Borne Bacillus cereus Strains". When I run gel...

27 March 2019 5,557 11 View

Anyone has worked or still working on detecting enterotoxin genes in B cereus?

Hi, Im working on detecting enterotoxin genes especially haemolytic enterotoxins (HBL) genes in B. cereus strains. I'm not able to detect the gene even from the positive control, ATCC 14579...

09 January 2019 9,718 0 View