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Questions related from Yugenraj Navaneethan
Hi I performed 24 hr biofilm formation assay (a total of nine replicates for each bacterial strain). And from the OD570nm, I classify them according to their biofilm forming capacity by using the...
23 August 2019 628 2 View
Hello Spoilage causing microorganisms (yeast, mold, bacteria) cause undesirable changes in food through their enzymatic actions. Is there a threshold value for enzyme activity (can be for any...
14 May 2019 7,743 1 View
14 May 2019 3,483 2 View
Hi I would like to know how to construct standard curve for azocasein assay? I know that tyrosine is usually used for the standard curve of protease assay. The tyrosine standard measured...
15 April 2019 9,785 3 View
Hi I have run gradient PCR to choose the optimum annealing temperature (picture attached below). In the picture, the amplicons (in red coloured box) obtained at 5 different annealing temperatures...
08 April 2019 8,260 6 View
I'm trying to detect hblA gene by using the PCR method as described by the journal"Enterotoxigenic Profiles of Food-Poisoning and Food-Borne Bacillus cereus Strains". When I run gel...
27 March 2019 5,626 11 View
Hi, Im working on detecting enterotoxin genes especially haemolytic enterotoxins (HBL) genes in B. cereus strains. I'm not able to detect the gene even from the positive control, ATCC 14579...
09 January 2019 9,760 0 View
