Specifically, I want to detect Salmonella enterotoxin gene and E. coli heat stable enterotoxin gene. However, I am confuse which PCR that I can use to detect those toxic genes. What primers should I use? And what PCR condition should I have?
Article Detection and Characterization of Shiga Toxin Producing Esch...
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Hi everyone. I have some confusions on ng of DNA and microliters (ul). I've read several PCR protocols which mentioned on the ideal concentration of DNA template per reaction. I couldn't...
02 March 2019 10,012 5 View
I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...
11 December 2018 9,698 4 View
I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...
11 December 2018 2,445 1 View
11 December 2018 5,041 1 View
Basically I want to look for the differences between storage periods and bacterial counts. However, I don't know which tests that I can use to determine the significant differences. For example,...
11 December 2018 9,111 4 View
11 December 2018 5,943 1 View
11 December 2018 7,012 1 View
11 December 2018 4,896 1 View
11 December 2018 5,363 0 View
11 December 2018 7,513 3 View
I have modelled a steel structure using beam elements in Abaqus and attached to this structure reinforced concrete slab. The analysis that I am making is heat transfer of the structure. The...
07 August 2024 1,028 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
is there any other way to heat a polymer evenly other than oil bath if so what are those and how to use it
06 August 2024 947 1 View
I want to Estimate surface heat fluxes using MyLake, but I don't have all the initial values in model parameters section and other sections,is there a way?
04 August 2024 1,537 1 View
A computational study of heat flux on cylindrical surfaces using CNT hybrid nanofluids
28 July 2024 4,985 1 View
I would like to conduct artificial sunlight experiments to mimic heat capture and air warming in a greenhouse system. The main objective is to heat up the temperature inside the greenhouse, not...
28 July 2024 4,006 2 View
I am an undergraduate mechanical engineering student seeking suggestions on topics related to either thermodynamics, fluid mechanics, heat transfer or renewable energy for my final year project....
24 July 2024 4,058 5 View
I make polycrystalline diamond with tape casting followed by HPHT sintering. When i sintered the samples up till May, the sintering was good. When i sintered the samples in June and July at the...
16 July 2024 3,212 5 View
Hello everyone, I am facing a consistent issue in my NMR spectra with an unwanted peak appearing at 1.25 ppm. This peak seems to vary with the amount of sample: it becomes more pronounced with...
15 July 2024 9,065 4 View
Heat transfer
06 July 2024 2,134 1 View