Specifically, I want to detect Salmonella enterotoxin gene and E. coli heat stable enterotoxin gene. However, I am confuse which PCR that I can use to detect those toxic genes. What primers should I use? And what PCR condition should I have?
Article Detection and Characterization of Shiga Toxin Producing Esch...
Article Detection of pathogenic Campylobacter, E. coli O157:H7 and S...
Hi everyone. I have some confusions on ng of DNA and microliters (ul). I've read several PCR protocols which mentioned on the ideal concentration of DNA template per reaction. I couldn't...
02 March 2019 8,907 5 View
I did PCR analysis for my samples (total 6 samples), I got bands for the 5 samples, however, there is one sample which the band is detected at 10,000 bp? What should I do? Is there any relations...
11 December 2018 9,557 4 View
I performed 16S rRNA PCR Analysis on a total of 6 samples. I used universal primers 27F and 1492R for this PCR analysis. 5 out of 6 samples show bands detected on 1500 bp marker, however there is...
11 December 2018 2,241 1 View
Basically I want to look for the differences between storage periods and bacterial counts. However, I don't know which tests that I can use to determine the significant differences. For example,...
11 December 2018 8,956 4 View
11 December 2018 4,841 1 View
11 December 2018 5,812 1 View
11 December 2018 6,855 1 View
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11 December 2018 7,276 3 View
Increased heat, drought and insect outbreaks, all associated to climate change, have increased wildfires. Decreasing water supplies, declined agricultural yields, health impacts in cities owing to...
01 March 2021 7,168 9 View
Should I mesh the solid domain ? or should I use wall thickness? or Shell conduction? for Heat Transfer between two fluids of a Heat Exchanger. what is recommended?
01 March 2021 9,785 3 View
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View
I have been trying to simulate heat transfer problem in Comsol 5.5 for long time but it always throw an error stating: "Failed to find a solution. Maximum number of Newton iterations...
28 February 2021 8,626 3 View
Hi Guys. I'm a mechanical engineer and have a bit trouble, would be very glad if you could help me with my questions. I have a pressurized tank which contains Natural Gas at 200 Bar and 293...
26 February 2021 6,253 3 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
I'm building a cfx model of thermoplastic pultrusion process on ansys and i have a doubt on how is the proper way to set this parameter in a porous medium
24 February 2021 1,915 1 View
I'm doing a cfx simulation of a multiphase flow in pultrusion and i set the Interfacial Area density in the heat exchange of a porous medium in this way IAD=Total fiber lateral surface of fiber...
23 February 2021 6,043 1 View
Hi I am working recently on a project where I have to study how the thermal properties or heat transfer enhancement of nanofluids as the volume fraction changes, or if a magnetic field is applied...
22 February 2021 961 2 View
Hello, I created a very tall tower (>1000m) and applied pressure boundary conditions at the inlet (bottom) and outlet (top) that correspond to the pressure are their relative altitudes....
22 February 2021 9,972 4 View