count mRNA to quantify the enterotoxin
Hi all, Currently, I electrodeposited polypyrrole on carbon electrode but I got some problem when polypyrrole also deposited on the surface of the counter electrode. The concentration of pyrrole...
05 February 2021 2,103 3 View
Dear Respectable Scientists, I have been trying to extract DNA from above mentioned sources using different CTAB/SDS Buffer Compositions and purifying agents but I am not able to extract DNA from...
12 January 2021 5,899 8 View
I have a dataset including Categorical variables and involves multiple response variable. 20% of the cells in cross tab has less than 5 frequencies and hence I cannot carry out a Chi-square Test....
26 December 2020 4,180 3 View
Hello fellow researchers around the globe :) Happy Holidays!! We are conducting a survey on understanding how device usage during everyday life has developed amid the Covid-19...
21 December 2020 5,363 4 View
I know that this system was regional before, thus covering specific areas not alla the global
09 December 2020 3,343 3 View
Educators around the world face a new challenge of how to conduct exams to assess what students have learned after the shift towards e-learning following the closure of nearly all universities...
21 September 2020 4,180 15 View
ith the development of Internet technology, online examination has become more Polar because it helps people save a great deal of energy and time. As an effective and efficient method of teaching...
21 September 2020 2,814 12 View
I have to calculate heat transfer co-efficient on shell side and plot its variation along the length of heat exchanger
07 September 2020 9,078 6 View
I am simulating shell and tube heat ex-changer i have mass flow inlet and pressure oulet (zero gauge) but my pressure readings are not accurate i have tried velocity inlet but results are same....
07 September 2020 7,298 11 View
hello dear microbiologist Am kinda new and still learning SPSS I want to know what kind of tests should I run for AST results I see lots of paper use chi square. And can anyone provide me with...
10 August 2020 9,911 4 View
Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...
03 March 2021 687 1 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...
02 March 2021 2,616 1 View
If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis
02 March 2021 7,670 3 View
For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...
01 March 2021 5,035 2 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...
28 February 2021 7,397 8 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
