Is enterotoxin detection in stool samples without cultivation possible?

04 March 2021 1 748 Report

I am looking for a method that enables Bacillus Cereus enterotoxin detection in human stool samples without prior cultivation. Direct detection that proves that enterotoxins were already produced in vivo is what I am searching for. Commercial immunoassays are available for enterotoxin detection but these require cultivation of Bacillus Cereus prior to detection. Is there a way to circumvent cultivation? Thank you!

Oscar L. Sierra

Possibly growth of bacteria is necessary to run the positive controls. If the vendor offers a purified extract, you buy it, and run your curve and samples with the standard curved prepared from an extract would be an option.

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