I tried ethanol 70% for 3 min, then bleach 5% for 3 min, followed by 3 rinses of sterile water. Also, tried the same method, but instead of 3 min used 6 min.
You might want to try mercuric chloride taking extra precaution related to exposure and disposal. Mercury is very toxic but workers have cited its high efficacy in surface disinfection.
I don't like to use mercuric chloride, as Paul say it's highly toxic. There are always other options less dangerous and, at the same time, effective as mercuric chloride. When I have to introduce in vitro materials highly contaminated, I prefer to give more a chronic than an acute treatment. What it does mean? As a first approaching, would reduce the concentration of chlorine and increase the disinfection time. At the same time, would reduce the pH of chlorine solution to increase the proportion of hypochlorous acid in it (during the process use masks to protect from gases!!!!!!). Always add some drops of tween or other detergent. Disinfection of highly contaminated materials is a hard work. Don't expect 100% of disinfection success, therefore use as many explant sources as you can manage. Good luck!!!!
Please also see the following link.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC439241/pdf/plntphys00313-0162.pdf
If the goal is to get seeds free of Fusarium moniliforme a better approach might be the infusion of the seed with benzimidazole fungicide. This would produce a high rate of seeds without fungal infection I believe.
you try only clorox and you don't need ethanol or try commercial hypo % 20 and 20 minute
Chlorine will have several issues such as it being a gas that attacks ozone and its direct toxicity. Iodophore would be a better alternative based on those considerations. Iodophore does not generate ozone attacking gas and is not so directly irritant on skin and tissue.
Iodophore disinfectant is so safe that it can be used to surface sterilize cow teats before milking without gloves. The coloration is useful to show the areas of interest were covered a useful indicator. Another very safe surface disinfectant is hydrogen peroxide which breaks down to oxygen and water. Initial developments in the field of disinfection did not consider some of environmental and health issues with these materials. Of the halogens Fluorine, bromine Chlorine and Iodine only Iodine can be defended on these grounds of environmental and health effects.
hola, bueno con el caso de los Yodóforos no tengo experiencia en uso (in vitro), en mi caso he usado el Dioxido de cloro como tambien el hipoclorito comercial... en algunos casos uso Cobox como tween 20.
Así mismo antes de todo lo lavo con un detergente comercial... luego cobox 1mg/L si deseas, despues etanol al 70% por un minuto o dos, seguido de Dioxido de cloro o hipoclorito comercial 2-4%, todo depende de la concentracion y el tiempo, en semillas similares he usado hipoclorito al 2%-3% con tiempos que pueden variar desde 10 a 20 minutos.
you use % 10 bleach and 10 minutes. it is very succesfuly other consantration (% 5). The corn seed is very resistant and unharmed
Paul Reed Hepperly, una consulta, con respecto a los yodoforos, acabo de conseguir uno cuya caracteristica es: Povidona yodada con 1g/100ml marca "ROKER". quería tú opinion y sugerencia de algunas marcas/productos de yodóforos.
por último, busque peróxido de hidrógeno pero no logre encontrar un producto que presente su concentracion (ppmm o g/L), los que vi solo presentan porcentajes (30%, 50%, etc), gracías de antemano.
- Badges
- Science topic