I don't know if somebody has nevere checked at DIFFERENT time points the fate of hNPC transplantated in mouse or rat. Could be suggested me some papers? Thanks in advance
I am conducting a systematic review and have an observational study. I want to perform some meta-analyses using the already calculated odds ratios. Can I use RevMan for this purpose? If not, do...
21 July 2024 4,110 4 View
Hey there, I am currently conducting a life cycle assessment and as advised, I used different life cycle impact assessment (LCIA) methods to check for robustness of results. I found that when...
03 June 2024 8,431 2 View
For a literatura review, I want to know the number of articles on my subject (school drop off) and the research doesn't provide that information
19 April 2024 5,232 1 View
Hello everyone, I've just realized that I haven't put the RNeasy MinElute spin column I received with the RNA extraction kit (Qiagen) in the fridge... I received them in December and I've just put...
16 January 2024 6,660 1 View
Hi all, im working on investigating epigenetic changes to a presynaptic transmembrane protein that seems to be important in PV neurons. I want to investigate neuron functional difference...
11 January 2024 7,305 3 View
I am running SDS page gels using the mini-PROTEAN tetra vertical tank. Gels ran normally up until about 3/4 of the way down the gel. At this point, the dye changes from blue to yellow and became...
09 January 2024 7,301 3 View
I have prepared a liquid medium for fermentation with kraft lignin in water, but the lignin was not completely dissolved. After fermentation, I am wondering about the best way to filter out the...
04 January 2024 7,479 1 View
I need to prepare a liquid culture medium for enzyme production. Kraft lignin will be the substrate in the medium. What would be the best solvent for this?
26 November 2023 7,130 2 View
Is there any difference between the protein extraction protocol from frozen cells and cultured cells?
20 November 2023 7,295 2 View
Hi all, I am trying to differentiate my IMR32 neuroblastoma cells using retinoic acid or a similar treatment, and was wondering if anyone had any protocols you've had success with that you would...
10 August 2023 8,042 0 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View
I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...
01 August 2024 7,199 1 View
What types of mask designs for metal deposition, to be used in a PVD system, are best suited for perovskite-based solar cells?
31 July 2024 4,835 3 View
Hi there! I recently started working on Lx-2 cells. When I thaw the cells, they start attaching within few hours but later in about 2 days they started rounding and dying. I used DMEM+2% FBS...
31 July 2024 3,893 0 View
Hi all, may I ask you for advice about treating monolayer culture cell line with essential oil. Due to the hydrophobic character of the oil, I am concerning how can it equally affect to all the...
31 July 2024 5,301 2 View