TC-2 Tissue sectioner SORVALL?

I bought this tissue sectioner SORVALL TC-2, but I do not find the manual and I would like to know if someone uses this machine and can help me to understand how it works.

01 December 2020 550 2 View

Can someone help me to understand IGEM 2016 protocol to Bacillus subtilis transformation?

I'm trying to transform Bacillus subtilis to fluorescence, and I will use this IGEM protocol with medium MNGE, but there's a part that I can't understand. It says: "Measure the OD600 of your...

29 October 2020 2,043 1 View

Carbon content over 100 in a charcoal sample under treatment at 1200 C?

After elemental analysis of the samples, carbon content of the charcoal was 102 (several repetitions were made, but obtained the same values), the equipment was tested with other samples of wood...

02 September 2020 1,971 2 View

Can anyone help me with my contaminated human muscle cells in culture?

I am facing a problem with cell culture for weeks now. The media of the cell culture turnes turbid (white little spots, see picture) but stays reddish/pink. First this happened after 2 weeks of...

01 September 2020 1,498 9 View

Chi-Square Goodness of Fit: Can I hypothesize an expected outcome of 0 for one variable and 100 for the other variable?

If you have three variables A, B, C and you want to test whether the hypothesis is that everyone focuses on C, can the expected outcome in a chi-square goodness of fit test then be A: 0 B:0 and C:...

28 May 2020 2,008 6 View

Does anybody have a good Cytoplasmic Membrane stainning protocol?

11 February 2020 6,922 3 View

Which antibody for mouse p300 IP?

Which is the best Antibody to perform IP of endogenous mouse p300 IP. Please provide a figure or a citation in your answer. Thank you!

24 September 2019 8,482 2 View

How can I compute a factor (latent variable) knowing the variables that contribute?

Dear all, I have a data set with 7 variables. I have run a EFA and I got three factors. I want to obtain a numerical value for each factor computed from the variables that form it but I am not...

20 January 2019 1,933 3 View

Any body working on spectroscopic investigations on functionalised fullerenes. experimental and theoretical approach

want to contact the person working in the field

08 July 2017 1,370 2 View

Negative Results on Nanodrop

I am having negative results for my DNA/RNA extraction from stool samples on Nanodrop for Concentration, 260/280 and 260/230. This is the first time I'm having this problem and have tried to use...

17 June 2017 4,774 12 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

No title

03 March 2021 5,360 2 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How can we raise the figure when we writing a paper with Latex ?

Hi, everyone. I'm a starter to write a paper by Latex. But I have some difficulty using it. I'm writing the paper with 2 columns. And, there is one figure that occupies the 2 both-sides. I want to...

26 February 2021 3,738 3 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Compute for the total volume of concrete needed for the concrete pouring of the foundation including its column up to NGL only as shown in the plan?

Looking for the quantity of cement, sand, and gravel of footings and columns. Thank you!

25 February 2021 3,092 2 View

No amplification data for qPCR on MxPro?

I have set up a qPCR run on MxPro with SYBR green and ROX as a reference, however, when I go to the analysis section the program seems to only collect data for the dissociation curves and not the...

25 February 2021 8,039 1 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Can I use TCA instead of TFA for protein analyis in RP-HPLC?

I am trying to analyze lipase using the reverse phase-HPLC C18 column. In the literature, 0.1 % TFA was and 60% ACN was used. Can I use TCA in place of TFA? If any other methods are available to...

25 February 2021 2,388 3 View