Anybody has suggestions for a good working in vivo model for metastatic neuroblastoma?

More Minu Samanta's questions See All

What kind of classification system to follow for categorizing video games ?

As a part of my research related to haptic experience I am trying to select video games as per genre. However, the online video game marketplaces have the genre scene quite confusing and no clear...

19 March 2024 7,972 4 View

What is my scopus i index ?

what is my scopus i index ?

24 January 2024 7,395 2 View

Is there any alternative to the ConSurf web server?

The website is temporarily down since mid-September 2023.

20 December 2023 8,416 0 View

What can I do to improve my ligation in molecular cloning?

I have an experiment, where I amplify a gene sequence with PCR and digest it afterwards with SalI and EcoRI. This gene sequence is the insert for my vector, which is also digested with SalI and...

23 October 2023 9,499 3 View

Who has used a Cell Cycle Analysis Kit?

Hi everyone, Please, who cloud tell me has used Cell Cycle Analysis Kit (MAK344 Sigma-Aldrich) and how was your experience? I will realice a in vivo experiment in oral ephitelial cell stimulated...

26 February 2023 3,238 0 View

How can I measure stress levels using the questionnaire model for a survey-based study?

What is the appropriate questionnaire design for a specific population?

20 January 2023 5,389 0 View

What is the acceptable range of bacterial endotoxin in purified recombinant in EU/ml?

Can anyone help me know what I should expect as an acceptable range of endotoxin in EU/ml from my purified recombinant protein sample, purified using E.coli expression vector.

21 November 2022 1,593 4 View

Why do Editors of Journal reject paper without sending it to the review ?

Its been occuring lately that a paper of mine is being rejected without entering the phase of being peer reviewed

09 June 2022 2,914 7 View

For vc relax calculations in quantum espresso under high pressure for (0-40 GPa), should i change the pressure value in the input file?

I am trying to do some vc relax calculations under high pressure(0-40 GPa) on LiC6. Should the 'press' value under the &CELL card be changed accordingly? What is generally the unit of pressure...

26 April 2022 9,072 1 View

What could be inferred from or how to interpret hierarchical agglomerative clustering of ward's method?

In a village level study with multiple criteria a hierarchical agglomerative clustering in ward's method with 3 groups of clustering has been performed in R, but the results show clustering among...

23 January 2022 4,600 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

What precautions should be taken while handling S. aureus enterotoxin Type B in the lab?

I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...

07 August 2024 6,034 3 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Saleh Alkarim

Hello

kindly look at links here

hope help you

Good Luck

https://academic.oup.com/carcin/article/26/3/513/2390765/Modeling-metastasis-in-vivo

https://www.ncbi.nlm.nih.gov/m/pubmed/22210712/

http://cancerres.aacrjournals.org/content/early/2016/11/29/0008-5472.CAN-16-1502

Minu Samanta

Thanks