Kazi Sarjana Safain

6 Questions 3 Answers 0 Followers

Questions related from Kazi Sarjana Safain

Can I leave fixed cells after transfection in 4°C fridge for four days prior to immunofluorescence staining?

Hi all I did transfection in 96 well plates and then fixed it with ice-cold 50%acetone and methanol, but could not proceed with immunofluorescence staining the wells for four days. Would it be...

22 February 2022 6,628 3 View

Any suggestions regarding IFA in 96 well plates?

Hello I am working on viral infection in PK-15 cells and then IFA in 96 well plates. After fixing the cells with 50%methanol and acetone for overnight at 40C and IFA, the wells turn completely...

22 December 2021 8,417 2 View

Name of some mastermixes which contains UNG enzyme?

Hello, We are planning to work with HBV viral load. I have read several articles on HBV viral load quantification and came to know that they have used such mastermix that contains UNG enzyme to...

17 September 2020 8,027 2 View

Why human plasma is used as HBV standard panels?

Hello, We are working on HBV viral load detection. However, most of the Hepatitis B virus molecular detection kits that are readily available uses direct human sample as HBV standard panel. So my...

17 September 2020 4,654 0 View

How to prepare 2x one step RT PCR mastermix?

Hello, I am working on developing a real time RT PCR based kit. For that, I need to prepare 2x one step RT PCR mastermix. I am using PCR Reagents and Verso cDNA synthesis kit from thermo...

04 May 2020 8,900 3 View

Is it necessary to use blood agar for antibiogram of gram positive bacteria? can i use Mueller Hinton agar instead?

Antibiogram of gram positive bacteria ( Enterococcus spp, Streptococcus spp and Staphylococcus spp. )

03 August 2019 4,575 11 View