What is the best protocol for genotyping of IR Flox mice?

Hello guys, I have a serious problem with genotyping of IR flox mice. First I did according the Jackson Protocol: https://www.jax.org/Protocol?stockNumber=006955&protocolID=16534 It should...

11 December 2019 1,825 3 View

Which chemokines are involved homing and accumulation of the activated CD8+ T cells in liver?

10 November 2015 1,191 3 View

Why do my Real time PCR proliferation plots look strange?

09 October 2015 8,465 11 View

What are small animals in mosquito salivary gland extraction beside the sporozoites??

31 December 2014 7,442 4 View

How can I generate memory 2C cells by hIL15 in vitro?

31 December 2014 4,792 3 View

How to activate CD8 T cells of 2C mice In vivo?

I want to activate CD8 T cells of 2C mice in vivo. CD8 T cells will be purified from 2C mice and then will be transferred IV to B6 mice. Next, splenocytes of Balb/C mice will be injected IV in to...

10 November 2014 1,260 3 View

Leishmania Major parasite burden.

Hello I used Real time PCR assay by L.Major and 3GPDH genes. 3GPDH is my hosekeeping gene. May any one help me in normalization of this RT PCR?

06 July 2011 2,393 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

What analysis to choose with a large neste dataset and clearly skewed or kurtotic distributions?

Hello! I have a dataset of n=3000 nested within 8 countries with approximately 200 or 400 responses in each country. I originally planned to perform multilevel modelling with 4 dependent variables...

02 March 2021 6,865 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View