8 Questions 16 Answers 0 Followers
Questions related from Masoud Akbari
Hello guys, I have a serious problem with genotyping of IR flox mice. First I did according the Jackson Protocol: https://www.jax.org/Protocol?stockNumber=006955&protocolID=16534 It should...
12 December 2019 1,887 3 View
Hi dears I am transferring in-vitro activated CD8+ T cells into host mice IV. They can enter the liver and accumulate around the infected hepatocytes expressing the antigen. I am wondering which...
11 November 2015 1,235 3 View
Hello everyone I have a problem with real-time PCR. My proliferation plots are strange recently. Please look at this picture: http://postimg.org/image/8e2pa9tg3/ or attached picture. The upper...
10 October 2015 8,513 11 View
I want to stimulate splenocytes of OTI and 2C mice by they cognate peptide invitro for 3 days to make them activated and proliferated. I tried it already with 2ug/ml OVA257-264 (SIINFEKL) peptide...
03 March 2015 7,630 0 View
Hello. I have a question. I want to generate 2C CD8 memory T cells by culture with hIL15. I need to generate very high number of these cells for cell transfer. I think my protocol is not good. I...
01 January 2015 4,838 3 View
I am getting Malaria sporozoites from mosquito salivary glands. we can see some sporozoites that are long and hardly moving and millions of small almost round animals that are around 1/10 of...
01 January 2015 7,521 4 View
I want to activate CD8 T cells of 2C mice in vivo. CD8 T cells will be purified from 2C mice and then will be transferred IV to B6 mice. Next, splenocytes of Balb/C mice will be injected IV in to...
11 November 2014 1,307 3 View
Hello I used Real time PCR assay by L.Major and 3GPDH genes. 3GPDH is my hosekeeping gene. May any one help me in normalization of this RT PCR?
07 July 2011 2,437 1 View
