What is the best protocol for genotyping of IR Flox mice?

Hello guys, I have a serious problem with genotyping of IR flox mice. First I did according the Jackson Protocol: https://www.jax.org/Protocol?stockNumber=006955&protocolID=16534 It should...

11 December 2019 1,825 3 View

Which chemokines are involved homing and accumulation of the activated CD8+ T cells in liver?

10 November 2015 1,191 3 View

Why do my Real time PCR proliferation plots look strange?

09 October 2015 8,465 11 View

Any suggestions on OTI and 2C splenocyte proliferation in vitro peptide concentration?

02 March 2015 7,583 0 View

How can I generate memory 2C cells by hIL15 in vitro?

31 December 2014 4,792 3 View

How to activate CD8 T cells of 2C mice In vivo?

I want to activate CD8 T cells of 2C mice in vivo. CD8 T cells will be purified from 2C mice and then will be transferred IV to B6 mice. Next, splenocytes of Balb/C mice will be injected IV in to...

10 November 2014 1,260 3 View

Leishmania Major parasite burden.

Hello I used Real time PCR assay by L.Major and 3GPDH genes. 3GPDH is my hosekeeping gene. May any one help me in normalization of this RT PCR?

06 July 2011 2,393 1 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Why tick's salivary gland extract didn't run in the gel (SDS PAGE )??

Greetings, I want to make SDS PAGE for my sample (Tick salivery gland extract), I tried concentrations 10%, 12%. I also tried high molecular weight ladder. But the protein stopped at the top and...

25 February 2021 7,698 3 View

Experimental Design: How deal with pseudreplicates with immunological and clinical-chemical paramaeters?

Hello everybody, I am investigating the influence of a toxinon various clinical-chemical and haematological parameters in fish. I have three treatments (control, low dose, high dose) in my...

24 February 2021 1,243 1 View

How does the injection volume impact LLOQ and ULOQ in regression analysis (LC-MSMS)?

Hello, I have run into an issue that has sparkled a debate at work: is the LLOQ/ULOQ impacted by injection volume under the following circumstances: 1) Same dilution factor of all samples 2)...

23 February 2021 9,159 3 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Extraction of DNA from insect samples conserved in silica gel?

Hi everybody, can anybody suggest how to extract DNA from insect samples conserved in silica gel (in my case, mosquitoes)? Does anybody know a commericial kit which does that or a particular...

10 February 2021 3,392 4 View

XRPD Quantification - Building a calibration curve of drug excipient?

I am aiming to utilise XRPD to quantify a drug excipient within a drug product and have been having a little trouble building the calibration curve to allow for this quantification up until this...

10 February 2021 6,145 3 View

The best RNA isolation kit for lncRNA RNA-Seq?

Hi, I am preparing a new project on lncRNAs in human cancer cell lines. I scanned through many high-impacted journals to find the best RNA isolation kit that would also isolate small RNAs and that...

09 February 2021 4,180 5 View