Leishmania Major parasite burden.

Masoud Akbari @Masoud_Akbari

07 July 2011 1 2K Report

Hello

I used Real time PCR assay by L.Major and 3GPDH genes.

3GPDH is my hosekeeping gene.

May any one help me in normalization of this RT PCR?

Masoud Akbari

Thank you Johannes

I had (Plasmid + cDNA) ctrl so I have the gene number of L.major and G3PDH and finally I used the ratio of L.Major/G3PDH.

As I know I should be acceptable.

Please tell me if you have any recommendation.

Thank you very much.

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Science method

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