I am in the middle of my project- expression of U-OMP16. I am very pressured for time, and cannot wait for a fresh sample of infected animal with brucellosis in order to isolate the Brucella strain and then the genomic DNA. I am going to clone the Protein portion of Omp16 (unlipidated Omp16 [U-Omp16]) to my plasmid , transfer it via agrobacterium to my plant and check the expression level. How can I solve this issue? Shall I order the gene that expresses this protein or try to get the required Brucella strain and then isolate the DNA from it ? What is the best way?
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