Monoclonal antibody
The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...
06 July 2019 7,192 4 View
After several protocols for gDNA isolation i have noticed that in all samples teh DNA is totally degraded. I have used several papers, some of them...
02 March 2017 9,339 7 View
Dear all, How to calculate the RP - resolving power of primer base on the band number on Gel? In the example figure (taken from one good paper) one primer has 7.12 RP. Can anyone tell me how...
08 September 2016 3,458 0 View
For the past two months I have been trying to analyse my AFLP regions on a 6% UREA PAGE GEL. The sample are selectively amplified with 4 set of primers.I have tried to optimize the protocols for...
11 December 2015 9,106 14 View
Dear all, I have transformed my Ecoli with a clone of size (14.3kb). Plasmid alone is about 13.9kb and insert of size 432bp. In 1:3 ratio I got around 200 colonies, negative control plate (only...
08 September 2014 2,289 5 View
I am in the middle of my project- expression of U-OMP16. I am very pressured for time, and cannot wait for a fresh sample of infected animal with brucellosis in order to isolate the Brucella...
11 December 2013 7,443 0 View
Plasmid - vectors
07 August 2013 9,081 3 View
Respected, Does anyone know how to construct a phylogenetic tree base on (PIC) and heterozygosity (H) values calculated for each SSR primer using Darwin software ? The PIC and heterozygosity...
01 January 1970 4,609 13 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...
31 July 2024 4,510 3 View
I am performing IgG purification and I have to show my results on SDS-PAGE. I use 10% tris glycine gel and prepare the samples under non reducing conditions. I am new to antibodies and therefore...
23 July 2024 6,664 6 View
I have immunized BalB/C mice with a protein using the intradermal (ID) method with Complete Freund's Adjuvant (CFA) and Incomplete Freund's Adjuvant (IFA), following a 14-day interval and three...
22 July 2024 9,160 2 View
Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...
21 July 2024 1,044 1 View
It was assumed that 4E8 T cells should be isolated and activated using plate-bound anti-human CD3, after which would be used for a genome-wide CRISPR screen. (Ref: Genome-wide CRISPR Screens in...
17 July 2024 3,371 0 View
Hi there, I'd like to use immunocytochemistry to determine a surface protein expression on mouse cell. The primary antibody I have is mouse anti mouse, and the secondary antibody I have is goat...
15 July 2024 6,008 5 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
Dear all, I'm going to radiolabel antibodies. So first of all, I'll remove excipients using the centrifugation method with amicon. Once I'm done, how do I calculate/measure the concentration of...
14 July 2024 3,106 3 View
