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Questions related from Jasmin Sutkovic
The primer annealing temperature , after the gradient PCR analysis, for the target gene differs from the optimal annealing temperature in housekeeping gene (positive control). What to do ? The...
07 July 2019 7,153 4 View
After several protocols for gDNA isolation i have noticed that in all samples teh DNA is totally degraded. I have used several papers, some of them...
03 March 2017 9,306 7 View
Dear all, How to calculate the RP - resolving power of primer base on the band number on Gel? In the example figure (taken from one good paper) one primer has 7.12 RP. Can anyone tell me how...
09 September 2016 3,425 0 View
For the past two months I have been trying to analyse my AFLP regions on a 6% UREA PAGE GEL. The sample are selectively amplified with 4 set of primers.I have tried to optimize the protocols for...
12 December 2015 9,045 14 View
Dear all, I have transformed my Ecoli with a clone of size (14.3kb). Plasmid alone is about 13.9kb and insert of size 432bp. In 1:3 ratio I got around 200 colonies, negative control plate (only...
09 September 2014 2,240 5 View
I am in the middle of my project- expression of U-OMP16. I am very pressured for time, and cannot wait for a fresh sample of infected animal with brucellosis in order to isolate the Brucella...
12 December 2013 7,407 0 View
Monoclonal antibody
09 September 2013 9,365 0 View
Plasmid - vectors
08 August 2013 9,046 3 View
Respected, Does anyone know how to construct a phylogenetic tree base on (PIC) and heterozygosity (H) values calculated for each SSR primer using Darwin software ? The PIC and heterozygosity...
01 January 1970 4,568 13 View
