Papers on protein-polyphenol-carbohydrate complexes?

I am looking for research on protein-polyphenol-carbohydrate complexes. I have seen a paper before that talked about higher complexes between proteins, polyphenols and certain carbohydrates that...

06 July 2019 5,959 1 View

Western blot - chemiluminescence interference?

Hey all, This is less of a question and more of a hint because I've been struggling with this for almost a year during my graduate career. In case you work with proteins and phenolic compounds...

02 March 2019 567 4 View

Where to publish technical note paper about a western blotting technique?

11 December 2016 6,968 0 View

Has anyone noticed high or uneven fluorescence background with iBlot PVDF membrane stacks?

I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...

04 May 2016 9,533 2 View

How useful/reliable is data obtained from quinoprotein staining by NBT (Nitroblue tetrazolium)?

09 October 2015 5,771 8 View

Is it possible to have ghost bands on immunoblot because polyphenols have been transferred with the proteins and block antibody binding?

Hello scientific world! I would appreciate your insight/experiences with the following: Has anyone else observed that polyphenols that have been complexed with proteins can (after SDS-PAGE) be...

03 April 2015 3,026 5 View

Protein sample (reduced, denatured, frozen) turns brown with increased storage time. Why is that?

My samples contain protein and likely green tea compounds. After my sample preparation I added NuPAGE reducing agent (Novex) and Novex NuPAGE sample buffer and heated the samples for 2 min at 85...

09 October 2014 4,138 5 View

Can urea (1M - 10M) in samples interfere or give false results for HPLC analysis of polyphenols (PACs in specific)?

06 July 2014 6,731 4 View

Is anyone using the EZQ protein quantitation assay? Urgent question about interference.

Hey, I am urgently looking for people that use the EZQ protein quantitation assay. It is used because it is one of the quick and easy methods to determine protein content with reduced interference...

04 May 2014 9,895 1 View

Why does the preliminary SDS-PAGE look funny after using 1 -10M urea to test protein stability?

I know I'll probably sooner or later find out what's going on but I would appreciate if some of you (having more experience with this than me) could help me out. I wanted to test the protein...

03 April 2014 7,633 12 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to calculate average trend in maximum annual NDVI from MODIS?

I am wanting to calculate the average trend in maximum annual NDVI in Iceland from 2010-2020 using MODIS MYD13Q1 V6. How would I do this? I have currently inserted the NDVI bands from the MODIS...

02 March 2021 752 2 View