Any suggestions where I could publish a technical note paper about a western blotting technique? I have already tried three outlets but my work is very specific to one technique.
I am looking for research on protein-polyphenol-carbohydrate complexes. I have seen a paper before that talked about higher complexes between proteins, polyphenols and certain carbohydrates that...
06 July 2019 6,074 1 View
Hey all, This is less of a question and more of a hint because I've been struggling with this for almost a year during my graduate career. In case you work with proteins and phenolic compounds...
02 March 2019 743 4 View
I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...
04 May 2016 9,649 2 View
I found that NBT can be used to specifically stain proteins that have been modified covalently by oxidized polyphenols (after electrophoretic transfer onto a nitrocellulose or PVDF membrane). All...
09 October 2015 5,886 8 View
Hello scientific world! I would appreciate your insight/experiences with the following: Has anyone else observed that polyphenols that have been complexed with proteins can (after SDS-PAGE) be...
03 April 2015 3,174 5 View
My samples contain protein and likely green tea compounds. After my sample preparation I added NuPAGE reducing agent (Novex) and Novex NuPAGE sample buffer and heated the samples for 2 min at 85...
09 October 2014 4,262 5 View
I was wondering if urea (1M - 10M) in samples can interfere or give false results when procyanidins are evaluated and quantified via HPLC?
06 July 2014 6,866 4 View
Hey, I am urgently looking for people that use the EZQ protein quantitation assay. It is used because it is one of the quick and easy methods to determine protein content with reduced interference...
04 May 2014 10,042 1 View
I know I'll probably sooner or later find out what's going on but I would appreciate if some of you (having more experience with this than me) could help me out. I wanted to test the protein...
03 April 2014 7,745 12 View
Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein? Or will they likely be broken off when sample buffer (and/or reducing agent) is added to sample and will not "be...
31 December 2013 2,061 6 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
I am Looking for a Science Journal with good impact factor and low publication cost to publish a review paper. Your suggestions would be appreciated.
06 August 2024 6,796 3 View
how to get links for copyrights for papers?
06 August 2024 7,410 1 View
I received an e-mail invitation to join the editorial board of Clinical Cardiology Updates. While I have published a few articles related to cardiovascular disease, there are lots of colleagues...
06 August 2024 8,981 8 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hello all, I wanted to know, can I use galaxy (USA, Europe or Australia) platform for analyzing the shotgun data, and can it be used for publication purpose as well? Thanks :)
06 August 2024 6,610 4 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I attempted to make a privately uploaded text public but a window appeared that said an error occurred. There was no explanation provided as to why there was an error or what might be done to...
05 August 2024 8,025 7 View