Papers on protein-polyphenol-carbohydrate complexes?

I am looking for research on protein-polyphenol-carbohydrate complexes. I have seen a paper before that talked about higher complexes between proteins, polyphenols and certain carbohydrates that...

06 July 2019 5,959 1 View

Western blot - chemiluminescence interference?

Hey all, This is less of a question and more of a hint because I've been struggling with this for almost a year during my graduate career. In case you work with proteins and phenolic compounds...

02 March 2019 567 4 View

Where to publish technical note paper about a western blotting technique?

11 December 2016 6,968 0 View

Has anyone noticed high or uneven fluorescence background with iBlot PVDF membrane stacks?

I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...

04 May 2016 9,533 2 View

How useful/reliable is data obtained from quinoprotein staining by NBT (Nitroblue tetrazolium)?

09 October 2015 5,771 8 View

Is it possible to have ghost bands on immunoblot because polyphenols have been transferred with the proteins and block antibody binding?

Hello scientific world! I would appreciate your insight/experiences with the following: Has anyone else observed that polyphenols that have been complexed with proteins can (after SDS-PAGE) be...

03 April 2015 3,026 5 View

Protein sample (reduced, denatured, frozen) turns brown with increased storage time. Why is that?

My samples contain protein and likely green tea compounds. After my sample preparation I added NuPAGE reducing agent (Novex) and Novex NuPAGE sample buffer and heated the samples for 2 min at 85...

09 October 2014 4,138 5 View

Is anyone using the EZQ protein quantitation assay? Urgent question about interference.

Hey, I am urgently looking for people that use the EZQ protein quantitation assay. It is used because it is one of the quick and easy methods to determine protein content with reduced interference...

04 May 2014 9,895 1 View

Why does the preliminary SDS-PAGE look funny after using 1 -10M urea to test protein stability?

I know I'll probably sooner or later find out what's going on but I would appreciate if some of you (having more experience with this than me) could help me out. I wanted to test the protein...

03 April 2014 7,633 12 View

Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein?

Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein? Or will they likely be broken off when sample buffer (and/or reducing agent) is added to sample and will not "be...

31 December 2013 1,947 6 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View