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Questions related from Nathalie Plundrich
I am looking for research on protein-polyphenol-carbohydrate complexes. I have seen a paper before that talked about higher complexes between proteins, polyphenols and certain carbohydrates that...
07 July 2019 6,021 1 View
Hey all, This is less of a question and more of a hint because I've been struggling with this for almost a year during my graduate career. In case you work with proteins and phenolic compounds...
03 March 2019 680 4 View
Any suggestions where I could publish a technical note paper about a western blotting technique? I have already tried three outlets but my work is very specific to one technique.
12 December 2016 7,039 0 View
I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...
05 May 2016 9,605 2 View
I found that NBT can be used to specifically stain proteins that have been modified covalently by oxidized polyphenols (after electrophoretic transfer onto a nitrocellulose or PVDF membrane). All...
10 October 2015 5,848 8 View
Hello scientific world! I would appreciate your insight/experiences with the following: Has anyone else observed that polyphenols that have been complexed with proteins can (after SDS-PAGE) be...
04 April 2015 3,117 5 View
My samples contain protein and likely green tea compounds. After my sample preparation I added NuPAGE reducing agent (Novex) and Novex NuPAGE sample buffer and heated the samples for 2 min at 85...
10 October 2014 4,222 5 View
I was wondering if urea (1M - 10M) in samples can interfere or give false results when procyanidins are evaluated and quantified via HPLC?
07 July 2014 6,825 4 View
Hey, I am urgently looking for people that use the EZQ protein quantitation assay. It is used because it is one of the quick and easy methods to determine protein content with reduced interference...
05 May 2014 10,001 1 View
I know I'll probably sooner or later find out what's going on but I would appreciate if some of you (having more experience with this than me) could help me out. I wanted to test the protein...
04 April 2014 7,706 12 View
Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein? Or will they likely be broken off when sample buffer (and/or reducing agent) is added to sample and will not "be...
01 January 2014 2,014 6 View
We have been using the above Western standard for Western blots but the standard never shows up after visualization with a chemiluminescent substrate (except in 1 or 2 cases actually). Has that...
12 December 2013 4,523 1 View
I have a random question about the EZQ protein assay. I just used it for the first time and really like it, it suits my test purposes well, as I have samples that contain pigments and phenolic...
11 November 2013 3,512 0 View
I am trying to resolve proteins that are smaller than 15kDa. Does anyone have suggestions for a gel-type and/or method?
09 September 2013 7,732 48 View
I am working qualitatively on an ATR-FTIR spectra of a high protein/high fat flour-majorly I am looking at modifications of Amide I and Amide II bands. Does anyone know what the band at around...
06 June 2013 3,726 19 View
