Western blot - chemiluminescence interference?

Hey all, This is less of a question and more of a hint because I've been struggling with this for almost a year during my graduate career. In case you work with proteins and phenolic compounds...

02 March 2019 567 4 View

Where to publish technical note paper about a western blotting technique?

11 December 2016 6,968 0 View

Has anyone noticed high or uneven fluorescence background with iBlot PVDF membrane stacks?

I was wondering if anyone else has used the iBlot PVDF membrane stacks and noticed uneven and/or relatively high autofluorescence / background when used for fluorescence Western blotting? I am...

04 May 2016 9,533 2 View

How useful/reliable is data obtained from quinoprotein staining by NBT (Nitroblue tetrazolium)?

09 October 2015 5,771 8 View

Is it possible to have ghost bands on immunoblot because polyphenols have been transferred with the proteins and block antibody binding?

Hello scientific world! I would appreciate your insight/experiences with the following: Has anyone else observed that polyphenols that have been complexed with proteins can (after SDS-PAGE) be...

03 April 2015 3,026 5 View

Protein sample (reduced, denatured, frozen) turns brown with increased storage time. Why is that?

My samples contain protein and likely green tea compounds. After my sample preparation I added NuPAGE reducing agent (Novex) and Novex NuPAGE sample buffer and heated the samples for 2 min at 85...

09 October 2014 4,138 5 View

Can urea (1M - 10M) in samples interfere or give false results for HPLC analysis of polyphenols (PACs in specific)?

06 July 2014 6,731 4 View

Is anyone using the EZQ protein quantitation assay? Urgent question about interference.

Hey, I am urgently looking for people that use the EZQ protein quantitation assay. It is used because it is one of the quick and easy methods to determine protein content with reduced interference...

04 May 2014 9,895 1 View

Why does the preliminary SDS-PAGE look funny after using 1 -10M urea to test protein stability?

I know I'll probably sooner or later find out what's going on but I would appreciate if some of you (having more experience with this than me) could help me out. I wanted to test the protein...

03 April 2014 7,633 12 View

Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein?

Can/do polyphenols "migrate" on SDS-PAGE/PAGE when previously bound to protein? Or will they likely be broken off when sample buffer (and/or reducing agent) is added to sample and will not "be...

31 December 2013 1,947 6 View

No title

02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

How Download/Extract citations of 1 given year ALL my publications in Google Scholar?

If you google this you find a lot of smart guys explaining you how to download 1 year of citations of 1 single paper of yours (or anyone else). I mean 1 year of ALL my papers. or ... ALL years or...

01 March 2021 1,121 9 View

How can I edit suggested publications?

Hi there! I regularly receive suggestion about papers and authors that are totally out of my area. Does anybody know how I can fix this? Thank you!

01 March 2021 9,574 4 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Any research paper call for publication related to management or finance or economics?

It should be standard journal (Indexed)

01 March 2021 8,379 1 View

Could anyone tell me what is the range of submillimeter wave?

There is confusion regarding the submillimeter-wave range. Many papers specify its range from 300GHz but some also consider its range from 100GHz. If I design an antenna on 122ghz what would I...

01 March 2021 516 7 View

Any research papers for extraction of nutrient deprivation on macroalgae? Analysed by HPLC-UV and GC/MS. Possibly from macroalgae genus Rhizoclonium?

I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...

28 February 2021 6,477 1 View