I found the Phenol / Chloroform protocol (i can't work with it) so I'm looking for the classical method.
Thank you !
Use Qiagen FFPE tissue kit or equivalent, but first treat the paraffin sections with tolyene (2 times) and rehidrate with 96% ethanol, followed by 70% and 50%, dry down, and start isolation with the kit.
thank you for your answer, i have already used it (Qiagen)but it takes a lot of time to get it from commercial so i'm looking for classical methode (proteinase K , lysis Buffer ).
From my experience the best method for small FFPE biopsy samples resulting in best quality was using the RecoverAll™ Total Nucleic Acid Isolation Kit for FFPE now from Thermo, previously from Life/Ambion (https://www.thermofisher.com/order/catalog/product/AM1975)
You can do TriZol if you don't want to use a commercial kit or in-house phenol-chloroform extraction.
However, here are just a few threads you can look through on RG to get an even more diverse opinion. Please see some of the following:
https://www.researchgate.net/post/What_is_the_best_DNA_extraction_method_for_FFPE_samples/1
https://www.researchgate.net/post/Has_anyone_isolated_RNA_DNA_and_proteins_by_TRIzol_in_FFPE_tissues
https://www.researchgate.net/post/Does_anybody_have_some_experiences_with_total_RNA_extraction_of_formalin_fixed_and_paraffin_embedded_FFPE_samples
other search results: https://www.researchgate.net/search.Search.html?type=question&query=ffpe%20dna%20extraction%20biopsy
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