I'm trying to design specific primers using Primer-BLAST of NCBI, the desired genes are sequenced and registered in the Genbank, but the software told me always that i couldn't get the specific primers, any suggession, please?
try primer 3 ................... or send me sequence i will try with geneious software ........ it may be better to design real time pcr primers and probe it is more easy.
Thank you very much for your reply also for for your help i'll try with Primer3 and even if it dosen't work with me i''l send you the sequenced gene, i hope that i'll not waist your time, one more time thank you very much, Younes.