Can someone suggest a suitable FTSC fluorescent label of proteins?

Could someone suggest a protocol for protein label, i'm using a protocol on which i wash the protein with ethylacetate-ethanol after Precipitation of protein with 20% TCA, i have a doubt that i...

10 November 2015 5,712 1 View

Any suggessions to learn how to design protocols for 2D electrophoresis gel?

For begginers in proteomics analysis how to learn about 2DGE, thanks in advance!

11 December 2014 9,318 0 View

Any suggessions for learning Bioinformatic tools?

Any suggestions for learning bioinformatic tools for beginners, any tutorial, website, training, workshop? Thanks in advence.

10 November 2014 8,900 11 View

Any body know about Mytibase?

I'm trying to access to the Mytibase, but it seems that there was some problems, since i get access on the site but but it look a static site and non functional, any suggessions? or any body have...

10 November 2014 6,288 4 View

Is there any other Database for sequenced genes else the NCBI genbank?

i'm looking for some sequenced genes gor Mytilus galloprovincialis, any help please?

10 November 2014 2,017 8 View

May I use Specific Primers from a specie sequenced gene for another specie?

I would like to measure the gene expression of some desired genes (in Mytilus), using RT-PCR, but those genes are not sequenced, I also found that the existence of the desired proteins, of those...

10 November 2014 6,562 7 View

How can I compare a sequenced gene from two or more differents species?

I would like to compare a gene (the same gene) from two different species, which software should i use, any suggessions ( software, tutorial...), i'll be grateful!

10 November 2014 8,375 6 View

Is it so easy to get in a field of research without write your name beside a known name?

is it true that some researcher (scientists) believe that a field is their own field, and every new researcher should stand on the door, requesting their permission, before his entrance ??? your...

10 November 2014 328 5 View

Any suggession for primers design?

I'm trying to design specific primers using Primer-BLAST of NCBI, the desired genes are sequenced and registered in the Genbank, but the software told me always that i couldn't get the specific...

10 November 2014 6,602 2 View

How much is useful scientific collaboration???

Scientific collaboration in the proper meaning of the word, supporting new idea without waiting for material benefits, is it really useful?

10 November 2014 7,237 12 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View