Dear Younes,

You can use the following:

Thermo Scientific Pierce Dye Removal Columns effectively bind to unconjugated fluorescent dye molecules from protein solutions to rapidly purify fluorescent conjugated antibodies and other proteins after labeling reactions.

These Fluorescent Dye Removal Columns enable fast and efficient removal of non-reacted fluorescent dyes from protein labeling reactions. Removing excess dye after a labeling reaction is often difficult and time-consuming but is essential for accurate determination of dye-to-protein ratios. The dye removal resin in this kit is highly specialized to produce exceptional protein recoveries while effectively removing non-conjugated dye. Using the appropriate amount of resin and buffer conditions, almost any fluorescent dye can be removed with this kit.

Features of Dye Removal Columns:

• Fast—removes free, unconjugated dye from labeled protein solutions in less than a minute

• Specific—purification resin provides outstanding conjugate recovery (75 to 95%)

• Customizable—separate resin and microcentrifuge columns allow optimization for different dyes and concentrations of sample

• Sample-friendly—processing results in minimal sample dilution

For more details please use the following link:

https://www.thermofisher.com/us/en/home/life-science/protein-biology/protein-labeling-crosslinking/protein-labeling/fluorescent-protein-labeling.html

Hoping this will be helpful,

Rafik