Dear naveed,

you can try the tools mentioned in the given link

http://www.phenosystems.com/www/index.php/links-to-various-tools-and-information/splice-prediction-tools

The above recommended site is great but I found another one that is little bit relevant to splicing defect prediction:

omictools.com/splicing-defect-prediction-category

 http://www.umd.be/HSF/

and

Thanks Dear all.

I know the splicing site but wana know which type of introns they may be. i.e type I , type II, type III or type IV intron.