Naveed Anjum

12 Questions 18 Answers 0 Followers

Questions related from Naveed Anjum

Does type of plasmid affect protoplast transfection efficiency?

i.e. GFP in different plasmid backbones but vector size same.

02 August 2023 740 4 View

Powdery mildew in maize: if does not infect the maize then what could be the role of MLO gene famiy?

I am talking with reference to the MLO gene family as in Barley where it gives resistance to powdery mildew.

02 May 2023 4,614 1 View

Does NOS (Nopaline Synthase) promoter work in Agrobacterium cells?

I am interested to know that does the selectable marker gene in the T-DNA region under NOS promoter will work in both plant and Agrobacterium cells. If yes, Kindly provide some reference ....

09 April 2018 2,128 19 View

How to amplify a GC-rich gene from genome?

i am amplifying a gene which is GC rich (5' highly GC rich ~76% and 3' ~66%). I have non-specific amplification but no desired band till now. I have done/used 1. gradient PCR 2. DMSO etc. but...

23 February 2017 1,646 5 View

Any software/online tool for prediction of intron splicing site and also type?

I have genomic sequences of a gene having putative annotation on ensemble. i want to know which type of introns it contain, i.e. type-I, II, III or IV. etc.

07 February 2017 4,176 4 View

which signal peptide should be used to target the (human or other) protein extracellular in transgenic plant? (secretory path like Bombyxin/insulin)

If i want to make a transgenic plant to express human protein or other, having secretory path just like Bombyxin or insulin then which signal peptide (plant origin ) should be attached So, that...

17 November 2016 5,460 0 View

Any constitutive promoter which give high expressio level also in endosperm?

there are many constitutive promoter but expression level in all tissue is not equal. any promoter which also give high expression level in seed and pollen? if any relevant paper then please...

07 November 2016 2,657 3 View

What points shoud be considered during codon optimization of glycosylated protein?

glycosylated protein. codon optimzation in such a way, that properly glycosylated and properly folded. what things should i ocnsider during codon optimzation

31 October 2016 9,346 3 View

Which sofware is best for codon optimization when optimize a gene of maize for cotton expression?

maize gene for cotton expression. codon optimization online tool or software.

24 October 2016 5,858 5 View

Is it possible to excrete active protease dirctly from Golgi coplexes which is ( zymogen ) glycosylated , proteolytic activated, and then secreted?

i have a pro-tease which is glycosylated and proteolytic activation is done before secretion to extracellular. is there any way to bypass all steps and directly target it to extracellular without...

21 October 2016 1,298 0 View

In CTAB DNA extraction method after grinding in CTAB, why it is incubated at 65 oC temperature for almost 30 minutes?

what is the reason for incubating it????

08 May 2015 3,387 5 View

Does GC-content of DNA fragment can effect migration on agarose gel?

two DNA fragment having same length but different sequence (i.e high GC and low GC-content). what about their migration on agarose gel? Thanks. Best Wihses.

01 January 1970 5,637 9 View