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Questions related from Abdul Nasir
Hello dears! I am doing a Co-Ip experiment while targeting an interaction of two sensitive proteins. How we can balance the total amount of proteins in Input, IP, IgG groups before the WB...
06 June 2023 6,467 0 View
Hello, I have two subunits (let say alpha and beta) between them a linker sequence (29 amino acid). I have modeled the alpha and beta subunit using templates extracted from PDB. There is no...
13 April 2017 9,552 11 View
Respected All, I am going to do the Ligand MD simulation using Justin Lemkul tutorial (http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin/gmx-tutorials/complex/03_solvate.html). After...
16 January 2017 4,342 3 View
Respected All, As through site-directed mutagensis, the catalytic activity of an enzyme is increase by analyzing there variants. But its time consuming and unpredictable when you are mutating the...
23 September 2016 5,333 3 View
i am going to model a protein (180 aa). when i blast the query sequence against PDB, it gives significant homology but coverage is >30%. I wanna to model whole protein structure, Although I...
01 June 2016 7,123 4 View
By using pymol, i am going to represent the surface of protein. It's is done when i click on the Show>surface. But i want to highlighted some residue above the surface of the protein (Like in...
18 January 2016 2,097 8 View
I am trying to start the MD simulation of the protein (energy minimized at 0.05) using MOE Docking software. But when I run the Dynamics, the software gives a failure that the time step is too...
26 October 2014 7,453 2 View
Hello, I have amino acid sequences of the same enzyme in more than 150 different organism which is grouped in two form i.e Aerobic and anaerobic enzyme. I did blasting of these sequences but i...
01 January 1970 9,154 1 View
