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Questions related from Abdul Nasir
Hello dears! I am doing a Co-Ip experiment while targeting an interaction of two sensitive proteins. How we can balance the total amount of proteins in Input, IP, IgG groups before the WB...
06 June 2023 6,518 0 View
Hello, I have two subunits (let say alpha and beta) between them a linker sequence (29 amino acid). I have modeled the alpha and beta subunit using templates extracted from PDB. There is no...
13 April 2017 9,607 11 View
As we know that at different PH, there will be different protein structure (Assembly) of a specific protein. I want to do homology modeling of the protein to consider the PH parameter in the...
04 March 2017 8,159 8 View
Respected All, I am going to do the Ligand MD simulation using Justin Lemkul tutorial (http://www.bevanlab.biochem.vt.edu/Pages/Personal/justin/gmx-tutorials/complex/03_solvate.html). After...
16 January 2017 4,428 3 View
Hello all, I am using PRODRG server for ligand topology file but in case of B12 as a ligand, contain different type of atom like Cobalt etc, when i submit the the input to the server it gives the...
16 January 2017 788 3 View
Respected All, As through site-directed mutagensis, the catalytic activity of an enzyme is increase by analyzing there variants. But its time consuming and unpredictable when you are mutating the...
23 September 2016 5,392 3 View
i am going to model a protein (180 aa). when i blast the query sequence against PDB, it gives significant homology but coverage is >30%. I wanna to model whole protein structure, Although I...
01 June 2016 7,187 4 View
Respected All, I have modeled a structure. when i am going to predict the quality of the modeled structure after energy minimization by Ramachandran plot. Some residues other than binding pocket...
29 April 2016 5,349 3 View
Respected all, i have identified six songs on the basis of spectrams analysis in the vocalization of Indian magpie robin . how can i interpret my audio format data for a bioacoutic journal.
04 March 2016 4,141 8 View
By using pymol, i am going to represent the surface of protein. It's is done when i click on the Show>surface. But i want to highlighted some residue above the surface of the protein (Like in...
18 January 2016 2,152 8 View
How to perform antioxidant activty of Blood serum on spectrophotometer after drug or extract treatment. please some one guide me.
13 March 2015 2,671 5 View
I am trying to start the MD simulation of the protein (energy minimized at 0.05) using MOE Docking software. But when I run the Dynamics, the software gives a failure that the time step is too...
26 October 2014 7,505 2 View
Hello, I have amino acid sequences of the same enzyme in more than 150 different organism which is grouped in two form i.e Aerobic and anaerobic enzyme. I did blasting of these sequences but i...
01 January 1970 9,209 1 View
