Also, how do I do DNA barcoding?
The pcr primers were nu-ssu-0817-5′ (ttagcatggaataatrraatagga), nu-ssu-1196-3′ (tctggacctggtgagtttcc), and nu-ssu-1536-3′ (attgcaatgcyctatcccca). Use this article for the primer sequence
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC92308/pdf/am004356.pdf
which of them is the best primer for determining fungi diversity by SSCP?
I'm going to do native page and i just want to know its principle completely.
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