Any recommendations for a good CRISPR/Cas9 commercial system for functional genomics of fungi/yeast?

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Seeking Software Recommendations for SELEX NGS Data Analysis?

I am looking for software to help analyze SELEX NGS data, including alignment, sequence enrichment, and other related tasks. Can anyone recommend suitable tools or software? Best wishes, Waleed

30 July 2024 1,061 5 View

Bootstrapping in SEM Amos ?

Can a researcher use small sample size of 40 while using SEM with bootstrapping of 1000 and is it possible to get published

28 July 2024 4,402 2 View

Are there any alternatives to PhenoScanner?

I am currently conducting a mendelian randomization study, and I was attempting to use PhenoScanner to look for potential confounders associated with the selected SNPs (any SNPs significantly...

23 July 2024 5,703 0 View

How do I access .vcf files without an R statistical package?

I am currently working on a mendelian randomization study, and I have downloaded the datasets needed from the ieu opengwas project (mrcieu.ac.uk) in .vcf format. I do not have access to an R...

19 July 2024 2,342 5 View

Correlation between two multiple indicator variables?

How to perform correlation analysis between two multiple indicator variables in spss?

16 July 2024 9,659 3 View

Where I could find research for type 2 fuzzy sets?

I want a research that explain how to add , subtract, multipy , divide type 2 fuzzy sets

28 June 2024 7,490 1 View

How can I get VegMeasure or SamplePonint Apps for Image Analysis of vegetation attributes?

I am looking for suitable application that make me fairly analyze image of vegetation taken by camera. such as VegMeasure or SamplePoint, I search this two Apps but I could not find the source of...

15 May 2024 7,558 0 View

How cal I share my profile link to Whatsapp ?

I need to share my profile with One else through WhatsApp, how can I do that?

23 April 2024 3,997 0 View

I am measuring cell viability of OD-21 cells and my hydrogels through Alamar Blue. When i calculate to get the Blank, i sometimes get a negative?

Why does a negative or a 0 for blank mean? Does this mean that cell viability has stopped?

27 March 2024 9,419 2 View

Does miller index change with doping?

Recently I'm working with Fe doped tin disulfide NPs. Without doping the index was perfectly matched with standard data but when iron was doped 2theta was changed approximately 2degree. now what...

26 February 2024 5,227 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you

08 August 2024 4,733 2 View

Is it possible to plot the atom-projected band structure using GPAW?

Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...

07 August 2024 269 3 View

Should I include H atom into C3N5 when i am doing DFT modelling?

Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...

07 August 2024 8,414 2 View

How much Yeast should we add to fermentation media?

I have prepared a liquid broth containing Yeast (S. Cerevisiae) that i need to add from it to fermentation media of lignocellulosic hydrolysate. I need to know based on what parameters do we add...

06 August 2024 662 1 View

Why did the authors extrapolate a phenotype that they experimentally proved in one bacterial strain across the whole genus of the organism?

I aim to be as skeptical as possible regarding whether a pair of orthologous genes results in the same phenotype in their different but related bacterial organisms under similar environmental...

05 August 2024 6,787 4 View

Are there any commercially available Donkey anti-Alpaca secondary antibodies?

Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...

04 August 2024 4,255 1 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

The Optimal Experimental Approach for Gene Function Analysis?

What is the most suitable experimental setting to understand the consequences of a particular gene: (1) targeted degradation of the specific transcription factor (TF) of the gene, followed by...

04 August 2024 6,265 1 View

What is fitofthora instance of potatoes?

Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.

02 August 2024 180 3 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View