Lauren Barbarich

6 Questions 8 Answers 0 Followers

Questions related from Lauren Barbarich

How do you analyze ELISA OD values to determine the best concetrations to work with?

I am developing and optimizing an ELISA and I was jsut wondering how you determine what the best concentrations of antibody and antigen are based off the OD values. I have performed checkerboard...

19 August 2019 7,741 3 View

What can be inferred from ELISA OD values that are higher for wells tested with no capture antibody versus wells with capture antibody?

I am trying to develop and optimize my own indirect sandwhich ELISA by performing cherkerboard titration. I am currently checking different dilutions of the capture antibody against the antigen...

09 August 2019 5,052 7 View

Where could I purchase lypholized Factor V powder so I can use it to create a standard curve in an ELISA?

I am developing my own ELISA for Factor V in mice, however I am having a hard time figuring out how I will be able to run a standard curve. I know people usually use a lypholized powder at a known...

29 July 2019 8,711 3 View

How do you set up a protocol on a plate reader for a checkerboard titration?

I am using a Gen 5 plate reader system and I am trying to set up a protocol to read a checkerboard titration for an indirect sandwich ELISA I am trying to develop. Would it be correct to define...

17 July 2019 9,755 1 View

Any reccomendations for an ELISA microplate washer?

I am looking into getting a plate washer for performing ELISAs to help with consistency and I was wondering if anyone had any reccomendations.

10 July 2019 291 3 View

What would be a good starting serial dilution scheme for a capture antibody and the antigen sample for an ELISA?

I am trying to develop and optimize an ELISA. To do so I will be performing a checker board titration to optimize what dilutions would work best. For the capture antibody I was thinking about...

17 June 2019 5,648 4 View