I am doing an experiment involving microinjections with zebrafish and have been experiencing high amounts of mortality.
The embryos look healthy upon injection, but many of them are dying between 24h and 120h post injection.
I've been playing around with lower temperatures and making sure fish aren't sitting too long in tricaine to reduce the amount of mortality post-injection, but neither seem to be changing much.
If anyone has any tips to possibly minimize the amount of fish that are dying, I would greatly appreciate it!
@all High mortality rates post-microinjection in zebrafish can be challenging, and troubleshooting the issues requires careful consideration of various factors. Here are some tips and considerations to help minimize fish mortality after microinjection:
If the issue persists, it may be helpful to consult with experienced researchers or the zebrafish community for specific troubleshooting tailored to your experimental setup. Additionally, considering the input of a zebrafish facility manager or veterinarian can provide valuable insights into optimizing the conditions for microinjection and post-injection care.
Hey there!
I have done zebrafish xenotransplantation before and I never had much mortality after injection. Some die off is expected and inevitable. What age are the larvae? In my experience, 48 hpf larvae seemed to survive ok when injected in tricane (maybe check your concentration, too high of a conc. can kill them), then washed in embryo water. I would then put them in fresh embryo water and put in the incubator at 33C. I used this temp because I was injecting human cancer cells and had to keep a happy medium between larvae temp and human temp. They never had any issue surviving at 33C.
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