Best approach to identify/prioritizing SNPs mediating a significant genetic correlation? What about genes/pathways/functions?

I am reporting some cross-phenotype genetic correlations (LDSC method) that are statistically significant after multiple test correction. Peer reviewers have asked us to report top SNPs that might...

31 December 2017 2,939 4 View

Do family-design and case-control GWASs arrive at the same answers? Any papers discussing actual/theoretical comparability or divergence?

02 March 2017 1,216 1 View

Is there a central database where one can look up CNVs in order to get a sense of evidence whether they might be pathogenic/affect brain development?

05 June 2016 944 2 View

Which staining approach is best (Golgi or alternative?) with pre-sectioned (40 microns) dendritic morphology/spine studies in fixed mouse brain ?

03 April 2015 5,076 6 View

Does ambient proton concentration (pH) alter the behavior of ROS/RNS?

02 March 2015 6,798 12 View

Has transcriptome-wide expression research ever translated into a clinically used biomarker or tool?

01 February 2015 2,145 6 View

Which is more vascular? Grey or white matter?

01 February 2015 5,971 0 View

Is there a tissue repository for post-mortem brain tissues obtained from subjects with post-traumatic stress disorder (PTSD)?

I'm wondering whether anyone is aware of a tissue repository where researchers can apply for access to post-mortem brain tissues obtained from subjects with post-traumatic stress disorder (PTSD)?...

08 September 2014 6,958 3 View

Any recommendations for a feature selection method that can be applied the same way across multiple kernel types (linear, radial, polynomial)?

Background: I have been working with SVM classifiers of microarray data for about 2 years. After a week-long search of the literature, I'm only beginning to appreciate the complexity of selecting...

05 June 2014 2,271 6 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Is it possible to have two luminescence bands of the same peak position but with different intensities in single spectrum??

Hi! If it is possible to have two luminescence bands of the same peak position in a spectrum, what is the reason behind formation of two separate bands instead of one? This phenomenon is related...

26 February 2021 4,963 5 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View