Daniel Tylee

10 Questions 5 Answers 0 Followers

Questions related from Daniel Tylee

Best approach to identify/prioritizing SNPs mediating a significant genetic correlation? What about genes/pathways/functions?

I am reporting some cross-phenotype genetic correlations (LDSC method) that are statistically significant after multiple test correction. Peer reviewers have asked us to report top SNPs that might...

01 January 2018 3,032 4 View

Any publications examining transcriptome (postmortem brain or blood, RNAseq or microarray) of ID/DD vs typical development?

Many transcriptome (genome-wide expression) studies have been carried out examining autism vs typical development using microarray or RNAseq in postmortem brain or ex-vivo blood. I know they...

03 March 2017 8,125 0 View

Do family-design and case-control GWASs arrive at the same answers? Any papers discussing actual/theoretical comparability or divergence?

I happen to be thinking about autism and other disorders with considerable SNP heritability. I'm curious to hear people's thoughts. Mine are below: One might assume the affected cases are...

03 March 2017 1,303 1 View

Is there a central database where one can look up CNVs in order to get a sense of evidence whether they might be pathogenic/affect brain development?

Not an expert on CNVs. I have a few samples from children with autism and their typically-developing siblings, which contain a few previously identified CNVs. Would like to get a sense if these...

06 June 2016 1,055 2 View

Which staining approach is best (Golgi or alternative?) with pre-sectioned (40 microns) dendritic morphology/spine studies in fixed mouse brain ?

I'll preface this post by indicating that I work primarily in bioinformatics, but I need to demonstrate some wet-lab skills in order to graduate with a degree in neuroscience. I'm proposing a...

04 April 2015 5,174 6 View

Does ambient proton concentration (pH) alter the behavior of ROS/RNS?

Let me preface this by saying I am not a biochemist. My logic may be very incorrect, but I'm looking for some guidance from someone who has a much stronger grasp on this field. Or if anyone...

03 March 2015 6,867 12 View

Which is more vascular? Grey or white matter?

I'm wondering if any generalization or educated guess can be made about vascular surface area per unit of cubic human brain tissue. Would the white matter or the grey matter have a larger...

02 February 2015 6,064 0 View

Has transcriptome-wide expression research ever translated into a clinically used biomarker or tool?

This is a big picture question for research historians among us. I'm wondering whether any gene expression microarray or RNA-seq study (or series of studies) has identified anything (a biomarker...

02 February 2015 2,215 6 View

Is there a tissue repository for post-mortem brain tissues obtained from subjects with post-traumatic stress disorder (PTSD)?

I'm wondering whether anyone is aware of a tissue repository where researchers can apply for access to post-mortem brain tissues obtained from subjects with post-traumatic stress disorder (PTSD)?...

09 September 2014 7,020 3 View

Any recommendations for a feature selection method that can be applied the same way across multiple kernel types (linear, radial, polynomial)?

Background: I have been working with SVM classifiers of microarray data for about 2 years. After a week-long search of the literature, I'm only beginning to appreciate the complexity of selecting...

06 June 2014 2,353 6 View