Working on ELISA : Any pitfalls i should be worried of.. Suggestion and improvements are welcome which can help my success in best reading in ELISA.
HPLC Column problems with Oils and Crude oil analysis.
01 February 2017 1,980 6 View
Hello, I am doing end point titer evaluation of serum antibody dilutions. As i am not using any standards or any sort of criterial to refer to as endpoint, can i use 3x of Ag Control values as...
07 August 2015 826 4 View
Hello Scientific Brains, In few of our studies in SDS PAGE, we are getting duplex band. Does this mean its a conjugated protein? of a subunit protein? Please
05 June 2015 7,771 9 View
Dear Friends, From past many days, i am getting problems with very high background reading for ELISA. 100ng Antigen Coated Skimmed milk 5% coating buffer having 0.5% tween Rabbit serum dilutions...
04 May 2015 550 15 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
Dear Colleagues and academy, I am conducting an action research study to understand the impact of phonological awareness activities using Quizlet and Boom on the reading comprehension skills of...
02 July 2024 8,480 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
"Adaptive Learning for Enhancing Reading Comprehension: A Case Study in Indonesian Junior High Schools"
08 June 2024 2,593 2 View