Vimarsh Gowda

5 Questions 39 Answers 0 Followers

Questions related from Vimarsh Gowda

Will a long chain fatty acid present in Crude oils will clog the HPLC column ? Will it be good to run Crude , Impure oils in HPLC..? any advise.. ?

HPLC Column problems with Oils and Crude oil analysis.

02 February 2017 1,960 6 View

Can I consider 3x of Ag Control values as End Point Titer in ELISA?

Hello, I am doing end point titer evaluation of serum antibody dilutions. As i am not using any standards or any sort of criterial to refer to as endpoint, can i use 3x of Ag Control values as...

08 August 2015 792 4 View

What does it mean to find a duplex band in SDS PAGE although markers are fine and crisp?

Hello Scientific Brains, In few of our studies in SDS PAGE, we are getting duplex band. Does this mean its a conjugated protein? of a subunit protein? Please

06 June 2015 7,745 9 View

Any pitfalls i should be worried of ? Suggest me the best practices to be followed with ELISA.

Working on ELISA : Any pitfalls i should be worried of.. Suggestion and improvements are welcome which can help my success in best reading in ELISA.

05 May 2015 2,138 0 View

Any suggestions on a high background for an ELISA reading?

Dear Friends, From past many days, i am getting problems with very high background reading for ELISA. 100ng Antigen Coated Skimmed milk 5% coating buffer having 0.5% tween Rabbit serum dilutions...

05 May 2015 510 15 View