I need suggestion from molecular biologist?

06 July 2016 2,088 2 View

Which method is cost effective for analysis of aflatoxin?

which is cost effective and time sparing method for detection of aflatoxin from food? ELISA, HPLC, or CE(capillary electrophoresis) Is CE is more efficient then ELISA.

06 July 2016 2,828 6 View

Are there any suggestions about E. faecalis infection in mice?

for bacterial infection i give 1* 10^9 cfu/ml (100ul) dose to mice,after 7 days mice are not infected.i need suggestion why mice r not infected. Because the article which i follow, in this with...

07 August 2015 6,091 2 View

Why does hexane dissolve in water during my flavonoid extraction?

During my reaction of extracting flavonoids I use hexane in a aqueous methanol crude extract to fractionate hexane and aqueous layer but surprisingly I don't get any layers. The hexane is...

10 November 2014 4,243 12 View

How do you fractionate methanol extract for flavonoids?

Subramanian and Nagarajan (1969). I'm using this protocol in this methanol extract fractionation, done by pet. ether, ethyl acetate and diethyl ether. Can I use n-hexane instead of pet. ether for...

10 November 2014 5,562 12 View

For DNA analysis CE 7100 Agilent can be used instead of genetic analyzer?

I am new user of agilent CE 7100. I worked on project on varietal identification and finding adulteration % of non basmati rice. For that I would use CE instead of genetic analyzer, is it...

01 January 1970 3,989 1 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View