I need suggestion from molecular biologist?

06 July 2016 2,088 2 View

Are there any suggestions about E. faecalis infection in mice?

for bacterial infection i give 1* 10^9 cfu/ml (100ul) dose to mice,after 7 days mice are not infected.i need suggestion why mice r not infected. Because the article which i follow, in this with...

07 August 2015 6,091 2 View

Why does hexane dissolve in water during my flavonoid extraction?

During my reaction of extracting flavonoids I use hexane in a aqueous methanol crude extract to fractionate hexane and aqueous layer but surprisingly I don't get any layers. The hexane is...

10 November 2014 4,243 12 View

How do you fractionate methanol extract for flavonoids?

Subramanian and Nagarajan (1969). I'm using this protocol in this methanol extract fractionation, done by pet. ether, ethyl acetate and diethyl ether. Can I use n-hexane instead of pet. ether for...

10 November 2014 5,562 12 View

For DNA analysis CE 7100 Agilent can be used instead of genetic analyzer?

I am new user of agilent CE 7100. I worked on project on varietal identification and finding adulteration % of non basmati rice. For that I would use CE instead of genetic analyzer, is it...

01 January 1970 3,989 1 View

Any one have sound knowledge on CE?

I am new user of capillary electrophoresis, is it possible to do varietal analysis by CE. Which capillary is required for DNA?

01 January 1970 8,684 1 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View