Why is the localization of protein in vivo is different from that of fixation?

Hi folks, I am recently working with Arabidopsis to look at the protein which has been reported to be at cell plate involved in cytokinesis with YFP tag. However, when I fixed seedlings which...

02 March 2017 8,478 3 View

A good anti c-myc antibody?

06 July 2016 4,287 5 View

Plugin for microtubule nucleation?

Hi folks, I have a problem to quantify microtubule nucleation. Is there any plugin of Image J to measure nucleation events automatically, since manual quantification takes long time and also...

04 May 2016 4,900 1 View

Does anybody know how to quantify microtubule bundlings?

Hi guys, I want to measure microtubule bundlings, but I have no clue how to cope with this. Do you know some plugins or methods? Thank you very much for your input! Cheers, Feng

08 September 2015 582 3 View

Can I use PBST in inmmunotaining?

07 August 2015 7,676 2 View

Any suggestions on the attempted GST tagged protein expression?

03 April 2015 4,153 7 View

How to prevent arabidopsis drifting under confocal microscope?

Hi everybody, recently, I encounter a problem of sample drifting under confocal microscope. As I want to record mitosis and cytokinesis which take 1 hour in total in arabidopsis roots, but...

02 March 2015 8,370 2 View

Why didn't FLAG antibody recognize FLAG fused N terminally protein in western blooting?

Hi everyone, FLAG was fused with my protein at N terminal. I performed immunostaining and could see fluorescence. However, when I checked its expression in western blotting, I could never detect...

01 January 1970 3,397 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

what is the simplest definition of ion exchange capacity

What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help

08 May 2021 0 0 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Which fluorescent dye to use for measuring total ROS production?

Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?

01 March 2021 9,447 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View