Hi guys,
I recently used anti c-myc antibody purchased from Sigma (C3956) to do whole mount immunostaining. However, the signal appered somtimes. In most cases, no signal. Does anybody know this? Which antibody do you use? Any suggestion is welcome.
Thanks a lot!
You may try the antibodies from the DSHB
http://dshb.biology.uiowa.edu/9E-10_2
But, often you have to work on the protocol to adapt to your specific needs. ICC stainigs are often a bit of trial and error.
Best,
Stephan
Hi,
no, not exactly this antibody, but a lot of others from the DSHB. As they are more or less non-profit, the antibodies are very cheap and worth a try. So far, I had good luck with all antibodies purchased from the DSHB.
Hi,
You can check a free antibody database at Labome.com. For c-myc antibodies, you can check the following link: http://www.labome.com/review/gene/human/c-Myc-antibody.html. For example, Santa Cruz has mouse anti-human monoclonal (9E11) and rabbit anti-human polyclonal (N-262) antibodies, suitable for IHC. Also, Invitrogen offers mouse anti-human monoclonal (9E10) to c-myc for IHC (reference: Ludwiq et al. Nature 2003). In addition, Abcam offers rabbit monoclonal (Y69) antibody (reference: Simeone et al. PLosOne 2014). I hope this comment was helpful.
I try to Express c-myc in mcf 7 cell line. Can any one give me the protocol for doing this expression and then western blotting ?
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