9 Questions 10 Answers 0 Followers
Questions related from Feng Lin
Hi everybody, I am wondering whether there is a membrane dye which is compatible with mCherry protein? Thanks a lot!
03 March 2018 5,446 0 View
Hi folks, I am recently working with Arabidopsis to look at the protein which has been reported to be at cell plate involved in cytokinesis with YFP tag. However, when I fixed seedlings which...
03 March 2017 8,540 3 View
Hi guys, I recently used anti c-myc antibody purchased from Sigma (C3956) to do whole mount immunostaining. However, the signal appered somtimes. In most cases, no signal. Does anybody know this?...
07 July 2016 4,355 5 View
Hi folks, I have a problem to quantify microtubule nucleation. Is there any plugin of Image J to measure nucleation events automatically, since manual quantification takes long time and also...
05 May 2016 4,961 1 View
Hi guys, I want to measure microtubule bundlings, but I have no clue how to cope with this. Do you know some plugins or methods? Thank you very much for your input! Cheers, Feng
09 September 2015 673 3 View
Hi guys, As I found very high unspecific background in immunostaining. I intend to dilute primary and secondary antibodies in PBST(tween-20)? In this case, does PBST affect antibodies...
08 August 2015 7,739 2 View
Hi guys, I am encountering a problem that my protein can't be purified by affinity purification, namely GST resin. This protein is a mutated protein where 20 amino acids were deleted. My protocol...
04 April 2015 4,208 7 View
Hi everybody, recently, I encounter a problem of sample drifting under confocal microscope. As I want to record mitosis and cytokinesis which take 1 hour in total in arabidopsis roots, but...
03 March 2015 8,448 2 View
Hi everyone, FLAG was fused with my protein at N terminal. I performed immunostaining and could see fluorescence. However, when I checked its expression in western blotting, I could never detect...
01 January 1970 3,456 0 View
