Any membrane dye compatible with mCherry protein?

Hi everybody, I am wondering whether there is a membrane dye which is compatible with mCherry protein? Thanks a lot!

02 March 2018 5,385 0 View

Why is the localization of protein in vivo is different from that of fixation?

Hi folks, I am recently working with Arabidopsis to look at the protein which has been reported to be at cell plate involved in cytokinesis with YFP tag. However, when I fixed seedlings which...

02 March 2017 8,478 3 View

A good anti c-myc antibody?

06 July 2016 4,287 5 View

Plugin for microtubule nucleation?

Hi folks, I have a problem to quantify microtubule nucleation. Is there any plugin of Image J to measure nucleation events automatically, since manual quantification takes long time and also...

04 May 2016 4,900 1 View

Does anybody know how to quantify microtubule bundlings?

Hi guys, I want to measure microtubule bundlings, but I have no clue how to cope with this. Do you know some plugins or methods? Thank you very much for your input! Cheers, Feng

08 September 2015 582 3 View

Any suggestions on the attempted GST tagged protein expression?

03 April 2015 4,153 7 View

How to prevent arabidopsis drifting under confocal microscope?

Hi everybody, recently, I encounter a problem of sample drifting under confocal microscope. As I want to record mitosis and cytokinesis which take 1 hour in total in arabidopsis roots, but...

02 March 2015 8,370 2 View

Why didn't FLAG antibody recognize FLAG fused N terminally protein in western blooting?

Hi everyone, FLAG was fused with my protein at N terminal. I performed immunostaining and could see fluorescence. However, when I checked its expression in western blotting, I could never detect...

01 January 1970 3,397 0 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Anyone know of a good progesterone antibody?

I am looking for a good progesterone antibody to quantify levels of progesterone in zebrafish. Applications would be ELISA, and immunohistochemistry.

28 February 2021 5,929 2 View

Will a 10xHis-tag prevent anti-MBP from binding to MBP?

I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...

25 February 2021 8,404 3 View