Hi guys,
As I found very high unspecific background in immunostaining. I intend to dilute primary and secondary antibodies in PBST(tween-20)? In this case, does PBST affect antibodies recognition?
Thanks a lot!
Leonardo Gatticchi
If you use an anti-phospho antibody it's better use TBST otherwise you can try with PBST.
To reduce the background you can adjust the tween 20 concentration (0.1% for example) or increase washes number/time.
Bye
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