Any inputs in doing time course experiments to check for miRNA effects post transfection in macrophages on regulation of pro-inflammatory cytokines?

04 April 2016 1 2K Report

miRNAs used: mimics for both 125b and let7B (will work with inhibitors also since this is a product of an endometriosis paper done previously)

transfection reagent: RNAimax

Volker Baumann

If you want to detect the alterations in the cytokine abundance I would suggest to perform an Elisa or Elisplot with your treated macrophages. This would be the best way to see if your treatment has a functional consequence on them.

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