Sepide Nematian

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Questions related from Sepide Nematian

Can i use MCF-7 cells and transfect them with miLet 7b to check for regulation of KRAS gene?

I wanted to do experiments on miRNA and its regulatory effects on target oncogene. miRNA Let7 family (7b in particular) has a negative regulatory effect on the KRAS gene. I want to prove this...

04 April 2016 8,385 3 View

Any inputs in doing time course experiments to check for miRNA effects post transfection in macrophages on regulation of pro-inflammatory cytokines?

miRNAs used: mimics for both 125b and let7B (will work with inhibitors also since this is a product of an endometriosis paper done previously) transfection reagent: RNAimax

04 April 2016 1,656 1 View