Does any one know how to get "publisher's acknowledgement of receipt for a submitted manuscript"? or the received email is enough?

I have to provide proof of submitted manuscripts especially those which are under review process.

06 July 2019 3,516 1 View

What is best method to protect one -NH of piparazine with benzyl bromide?

we want to protect one free -NH of piprazine with benzyl bromine using K2CO3 in CH3CN. any suggestion recommendation is appreciated.

03 April 2019 2,444 2 View

What is best Mass spectrometry book with detail description of all types of mass analyzers?

Mass analyzers in Mass Spectrometry

09 October 2018 4,245 3 View

How we can calculate no of rotatable bonds in an organic molecule?

is there any free software for this purpose, can we do it by Chemdraw.

06 July 2018 5,807 3 View

Can anyone please suggest me a very comprehensive yet advanced book on natural produts covering introduction, isolation and major classes?

if possible please send me the pdf of book

31 December 2017 8,574 4 View

Can anyone suggest why reaction of tetrahydropyridine-2,3-dicarboxylate with hydrazine hydrate isn't successful in refluxing EtOH or MeOH?

31 December 2015 7,792 15 View

AgOTf or AgSbF6 which is more strong lewis acid?

Which one acts as more strong Lewis Acid?

08 September 2014 4,719 4 View

Does aniline stick in columns during column chromatography?

I made schiff base of carbazole aldehyde using excess of aniline, now I need to purify the product. I was just wondering will my schiff base remain stable at column and aniline will come first, or...

07 August 2014 1,298 8 View

What is the best method for synthesis of primery amines by gabreil synthesis?

Does it need acidic workup, or simple extraction with some organic solvent is OK. I already have this paper but some literature say we need acidic work up for complete removal of pthalylhydrazide...

08 September 2013 6,795 7 View

Can someone advise on whether 1-bromomethylpyrene is light sensitive or not, and does it degrade on silica?

My 1-bromomethylpyrene is pure by 1H, 13CNMR and GC-MS, but when ever I take Tlc it gives 4 or 5 spots. I am unable to understand. There is not a single extra peak in 1H and 13CNMR.

06 July 2013 5,589 2 View

What element is driving the different slop in the low frequency tail?

My study consists simple corrosion inhibitors in salty brine. Right now I just fit my circuit with randles with CPE. Just wondering how the slop can given more info of the experiment. Or any...

01 March 2021 6,474 3 View

Can I use a protein-devoid condition as my negative control in ELISA?

Hello, I am running a sandwich-type ELISA where I am trying to inhibit a protein-protein interaction using small molecules. For each experiment, I determine my Z' (Z-prime) to determine the...

23 February 2021 7,429 1 View

How to calculate the fraction metabolized of a drug by an enzyme using clearance(ml/min) values of the metabolite formed?

I am trying to find the fraction metabolized of a drug by CYP and UGT enzymes. I am unable to get the values of fraction metabolized (Fm) directly from the literature. Can we calculate the Fm of a...

21 February 2021 5,379 2 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Phenol-pH graphic?

We want to use phenol pH graphic to measure the pH of mediums before and after inhibitor application to cells. We have phenol absorbence values at 460, 560 and 720 nm wavelength. When we measure...

20 February 2021 7,700 3 View

Which way will be better to give OGT inhibitors doses through intravenous or orally for pregnant female rats and concentration suggestion?

I'm planning to use ST078925 OGT inhibitor from TimTec (OGT-O-linked N-acetylglucosamine (GlcNAc) transferase) for female rats.

16 February 2021 7,850 2 View

How long does it take to design and validate transition state analogue inhibitors?

Hi, I'm asking from the point of view of designing a research plan: How long (approximately) can it take to determine the transition state structure of an enzyme substrate using kinetic isotope...

14 February 2021 2,610 1 View

Kinetics of Enzyme production in Recombinant E Coli?

Hello, I am trying to find kinetic information (such as rate of enzyme production etc.) on the expression of taq polymerase in E-Coli after inserting a recombinant plasmid. Any help would be...

12 February 2021 1,386 3 View

No title

Hello! I want to determine the activity of my in house Reverse Transcriptase enzyme. For this, I have used Thermo's Enzcheck Reverse Transcriptase assay kit. I used commercial Thermo RT enzyme to...

08 February 2021 7,285 1 View

Has anybody administered MK-2206 (an orally active, allosteric inhibitor of AKT) intravenously to the mice?

We aim to use MK-2206 to inhibit AKT in mice. However, a large body of studies suggests that it should be administered orally. I was wondering if somebody could tell me that is it toxic for the...

07 February 2021 6,340 1 View