I want to transfect pGL4 plasmids in epithelial cells.
After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just...
31 December 2019 7,829 2 View
I want to encapsulate miRNAs into liposomes, and it doesn't seem to work with my mini-extruder, so I am ready to use the service of a competent company. Any suggestions?
31 December 2019 8,903 2 View
Exiqon, we don't need a transfection agent! Any thoughts?
09 October 2016 1,688 2 View
I have isolated exosomes using a kit, but diluted it in PBS too much, I need it more concentrated for cell treatment. Any suggestion on how to concentrate the exosomes with and without...
05 June 2016 2,756 3 View
I have hundreds of samples and could not find a useful software or application that I can use.
08 September 2015 667 0 View
for example I have pure exosomes with average size of 51nm and with a concentration of 230.5E8 particles/ml. Can I get a concentration of the "proteins" levels?
01 January 1970 4,124 1 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...
08 August 2024 4,645 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I need to introduce an ARS (autonomously replicating sequence) in my plasmid but I'm not sure which position would be the best. Does anyone have any suggestion? A picture of the plasmid...
05 August 2024 1,573 4 View
I want to introduce a point mutation (change in one nucleotide) into my gene of interest (DNA binding domain) I have designed primers as recommended on the Data sheet of the kit : -Both primers...
05 August 2024 9,059 3 View
I am performing ligation of the plasmid and a target gene. The steps I have taken are: 1. Double digestion of the plasmid and target gene 2. Ligation of the plasmid with the target gene 3....
05 August 2024 2,570 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View