What reliable company can encapsulate specific microRNAs into liposomes as a service?

I want to encapsulate miRNAs into liposomes, and it doesn't seem to work with my mini-extruder, so I am ready to use the service of a competent company. Any suggestions?

31 December 2019 8,781 2 View

Can we extract exosomes from stool using the Norgen Urine Exosome Purification kit?

After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just...

31 December 2019 7,685 2 View

MiRNA mimic and inhitors... Exiqon or invitrogen?

Exiqon, we don't need a transfection agent! Any thoughts?

09 October 2016 1,603 2 View

How to concentrate exosomes?

I have isolated exosomes using a kit, but diluted it in PBS too much, I need it more concentrated for cell treatment. Any suggestion on how to concentrate the exosomes with and without...

05 June 2016 2,618 3 View

Any suggested ONLINE-FREE application/software for the analysis of relative gene expression using Ct values on real-time PCR?

I have hundreds of samples and could not find a useful software or application that I can use.

08 September 2015 562 0 View

How can I know the exosomes concentration out of the "particle per ml" nanosight data?

for example I have pure exosomes with average size of 51nm and with a concentration of 230.5E8 particles/ml. Can I get a concentration of the "proteins" levels?

01 January 1970 4,042 1 View

What is the most Operating System (OS) which can be INSTALL on Tiny hard devices like Raspberry Pi (RPi) hardware ?

I'm targeting to deploy a mesh network and manually configure MANET routing protocols. I'm preparing scenarios, architectures, and hard devices needed to do that. Are there some step-by-step...

03 March 2021 1,931 5 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How can I integrate a routing protocol in a tiny device (e.g. Raspberry Pi)?

Hi Community, I'm facing the issue of integration/compiling a new routing protocol in a WSN simulator. The final goal is to successfully add, configure this routing protocol in hardware devices...

01 March 2021 9,332 6 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View