Adel Driss

7 Questions 2 Answers 0 Followers

Questions related from Adel Driss

Can we extract exosomes from stool using the Norgen Urine Exosome Purification kit?

After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just...

01 January 2020 7,773 2 View

What reliable company can encapsulate specific microRNAs into liposomes as a service?

I want to encapsulate miRNAs into liposomes, and it doesn't seem to work with my mini-extruder, so I am ready to use the service of a competent company. Any suggestions?

01 January 2020 8,861 2 View

Any good simple protocol for reverse transfection using lipofectamin 2000?

I want to transfect pGL4 plasmids in epithelial cells.

12 December 2016 2,636 0 View

MiRNA mimic and inhitors... Exiqon or invitrogen?

Exiqon, we don't need a transfection agent! Any thoughts?

10 October 2016 1,664 2 View

How to concentrate exosomes?

I have isolated exosomes using a kit, but diluted it in PBS too much, I need it more concentrated for cell treatment. Any suggestion on how to concentrate the exosomes with and without...

06 June 2016 2,719 3 View

Any suggested ONLINE-FREE application/software for the analysis of relative gene expression using Ct values on real-time PCR?

I have hundreds of samples and could not find a useful software or application that I can use.

09 September 2015 628 0 View

How can I know the exosomes concentration out of the "particle per ml" nanosight data?

for example I have pure exosomes with average size of 51nm and with a concentration of 230.5E8 particles/ml. Can I get a concentration of the "proteins" levels?

01 January 1970 4,094 1 View