How to concentrate exosomes?

More Adel Driss's questions See All

Can we extract exosomes from stool using the Norgen Urine Exosome Purification kit?

After dilution of Fecal matter in PBS, the technician used the Noregen Urine Exosome Purification kit and RNA extraction. So my basic question is: Do you think we've got actual exosomes or just...

31 December 2019 7,829 2 View

What reliable company can encapsulate specific microRNAs into liposomes as a service?

I want to encapsulate miRNAs into liposomes, and it doesn't seem to work with my mini-extruder, so I am ready to use the service of a competent company. Any suggestions?

31 December 2019 8,903 2 View

Any good simple protocol for reverse transfection using lipofectamin 2000?

I want to transfect pGL4 plasmids in epithelial cells.

11 December 2016 2,673 0 View

MiRNA mimic and inhitors... Exiqon or invitrogen?

Exiqon, we don't need a transfection agent! Any thoughts?

09 October 2016 1,688 2 View

Any suggested ONLINE-FREE application/software for the analysis of relative gene expression using Ct values on real-time PCR?

I have hundreds of samples and could not find a useful software or application that I can use.

08 September 2015 667 0 View

How can I know the exosomes concentration out of the "particle per ml" nanosight data?

for example I have pure exosomes with average size of 51nm and with a concentration of 230.5E8 particles/ml. Can I get a concentration of the "proteins" levels?

01 January 1970 4,124 1 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View