I have to use 3-MA in my cell culture at high concentration. II know that 3-MA is not very soluble in water, so the solution would be solubilize 3-MA in DMSO, but I know that DMSO generally alters the phenotype of cells in culture.
Any suggestions?
I don't know which concentration did you use (how high) ? We usually dissolve 3-MA in
hot water (nearly 100 ℃), and then adding 3-MA solution in different culture medium. We usually use 2mM of 3-MA.
I have to use it 7 mM final concentration.... what's the concentration of your stock solution'
Heat it up to 100C in H2O was what I have used and I agree with Dr. Li.
Hi everyone, I'm isolating microglia from P1-2 mice and seeding microglia cells from 3 pups brains/T-25 flask. After seeding, I let this astrocytes-microglia mix culture grow for approx 2-3 weeks,...
06 October 2020 3,916 3 View
I need to test the stimulation production of TNF alpha in the RAW cell line by LPS. I am looking for an easy and cheap experiment to quantify the serial dilution stimulation in this macrophage...
27 September 2020 5,366 6 View
Hello! I am looking for good antibodies that help in clearly distinguish between D1- and D2-type medium spiny neurons (MSNs) in the striatum, by immunohistochemistry and immunofluorescent...
31 May 2020 5,009 3 View
Hello! Do you know some good antibodies, suitable for IFL, for differently staining the M2 and M2 microglia phenotypes in rat tissue? I am searching for antibodies specific for RAT because all the...
27 October 2019 7,260 3 View
I need to buy analytical standards for grayanotoxin I, II and III. I only find the type I. Roberta Merlanti
15 May 2019 2,628 4 View
Thanks!
06 October 2016 4,648 3 View
Tetrakis Hydroxymethyl Phosphonium Sulphate and methanogenic bacteria
21 September 2014 8,375 2 View
Do more people use cDNA, siRNA, mRNA or miRNA? Would love to hear what people are using?
29 January 2014 1,147 7 View
cDNA, mRNA, siRNA, miRNA
27 January 2014 4,591 3 View
Our patient is refractory after 3 lines of ICANS post KTE X19 treatment for MCL (dexamethasone, methylprednisolone, anakinra). Has anyone used Dasatinib for neurotoxicity in post CAR T cell use?...
01 January 1970 2,854 1 View
Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1
17 August 2021 0 0 View
20 June 2021 0 0 View
--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?
24 March 2021 0 0 View
Beginner level books. High level books. Analytical Number Theory. Algebraic Number Theory. Combinatorical Number Theory. Books about partitions. Books about primes.
03 March 2021 7,174 5 View
What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...
03 March 2021 1,401 3 View
dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...
03 March 2021 6,994 5 View
I looking for ferro calcium production process
03 March 2021 1,755 4 View
Hello, good day! I am trying to simulate similar bimorph piezoelectric harvester with below commands. However, the output power doesn't change with resistor value. Please help to find the reason. ET,10,CIRCU94,0 !SET UP RESISTOR R,1,100000 !RESISTANCE VALUE TYPE,10 !SET ELEMENT TYPE E,1,2 !CREATE RESISTOR BETWEEN NODES 1 AND 2 Thanks in advance Regards,
02 March 2021 0 0 View
Hello, I'm fairly new to ARENA and I have the following problem: I have two queues in which, in each queue, a different order type arrives (both having an arrival rate of exponential 10). Order...
02 March 2021 2,431 3 View