I have to use 3-MA in my cell culture at high concentration. II know that 3-MA is not very soluble in water, so the solution would be solubilize 3-MA in DMSO, but I know that DMSO generally alters the phenotype of cells in culture.
Any suggestions?
I don't know which concentration did you use (how high) ? We usually dissolve 3-MA in
hot water (nearly 100 ℃), and then adding 3-MA solution in different culture medium. We usually use 2mM of 3-MA.
I have to use it 7 mM final concentration.... what's the concentration of your stock solution'
Heat it up to 100C in H2O was what I have used and I agree with Dr. Li.
the molecule name is citated in old literature about organic sunscreen molecules
10 July 2024 5,576 2 View
Good morning, I urgently need to perform ddPCR analysis on some samples using a QX200 Droplet Reader that's been sitting in my lab for a while. A technician came in last month to set it up and I...
09 April 2024 4,115 2 View
Hi everyone, I will soon start setting up the cuprizone model of demyelination/multiple sclerosis. Cuprizone is purchased from Sigma in powder form and mixed with regular rodent chow (0.2% w/w)....
09 January 2024 4,302 0 View
O termo “conforto” pode ser usado para descrever um sentimento de contentamento, uma sensação de aconchego, ou um estado de bem-estar físico e mental. Questões relacionadas ao conforto foram e são...
27 December 2023 9,271 0 View
I have a fixed effects model with only few observations and would therefore like to bootstrap in order to obtain more accurate standard errors. At the same time, I assume SE to be clustered thus I...
21 December 2023 6,797 0 View
We also tried to identify peptides from the yeasts used during the fermentation process and for such we tried to apply the Saccharomyces sp. and other Saccharomyces protein databases from UniProt....
09 November 2023 5,863 1 View
Can you suggest me an innovative empirical research on the capital structure of multinational firms? Anyway, thank you.
23 October 2023 9,184 4 View
'in particolar modo vorrei trovare articoli inerenti al comportamento di alcuni organismi marini in funzione del rumore antropico.
17 July 2023 3,728 0 View
exploring potential instrument for organic carbon and carbonate analysis
11 May 2023 3,661 1 View
I potentially could follow the same steps as to calculate lethal concentration 50. The steps for Prism are outlined here: https://www.graphpad.com/support/faq/how-to-determine-an-icsub50sub/ I...
06 March 2023 4,876 2 View
"PUBLISHING IN A SCOPUS JOURNAL" Researchers are now at a cross road. The critical need to publish in a Scopus or ISI, etc journal is ever vital. Journal Publication fees must be submitted....
10 August 2024 8,621 1 View
Hello everyone, I am currently developing a thesis proposal and would appreciate your input on its viability and how to effectively carry it out. My proposed topic is: "Does the perceived threat...
10 August 2024 8,992 0 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Who will bear moral responsibility for the deaths of thousands of people in the event of an earthquake? Weeks and months remain before the onset of strong earthquakes that bring death to...
08 August 2024 6,134 12 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I'm currently working on a project where I need to plot the atom-projected band structure using GPAW. I've been able to calculate the band structure for my material, but I'm having trouble...
07 August 2024 269 3 View
Hi all, my experimental XPS results shown that my C3N5 sample consists of N-H bond, hence in this case I should incorporate the N-H bond into my DFT modelling. However, I do notice several papers...
07 August 2024 8,414 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View