Let's say I have 5-time points (12h, 24h, 48h, 72h, 96h ) and 5 temperatures (20, 22,24,26,28C); then I would calculate LT50 for each time point

Calculate LT50 with Prism.

Can I use Prism to calculate Lethal Temperature 50 (LT50)?

Prism is a powerful software tool that can be used for a wide range of scientific analyses, including the calculation of LT50 values. LT50 is a common measure of thermal stress in organisms, and Prism includes several methods for calculating this value.

To calculate LT50 in Prism, you can use the "Dose-Response/Pain Analyses" feature. This will allow you to plot the response of your organism to different temperatures, and then fit a curve to the data to estimate the temperature at which 50% of the organisms are expected to die.

Here are the steps to follow:

Enter your data into Prism. You should have a column for the temperature and a column for the proportion of organisms that survived at each temperature.

Go to the "Dose-Response/Pain Analyses" tab and select "Survival analysis" from the dropdown menu.

Select the appropriate model for your data. For example, if your data follow a sigmoidal curve, you may want to select the "Logistic" model.

In the "Analysis Settings" dialog box, make sure to select the "LT50 (temperature at which 50% survival is expected)" option.

Click "OK" to run the analysis. Prism will fit the selected model to your data and calculate the LT50 value.

You can view the LT50 value in the results table, and also plot the curve and the LT50 value on your graph.

Note that the method used to calculate LT50 in Prism may depend on the specific model and settings that you choose, so it's important to understand the underlying assumptions and limitations of your analysis.